The above figure shows where sample applied was 100 µg of total protein extracted from a normal dog heart ventricle. The first dimension was conducted using a pH 4–7 isoelectric focussing gel. The second dimension was a 12% SDS–PAGE vertical slab gel. The pattern was visualised by silver staining. Which of the following techniques is applied

#Question id: 10276

#Part-B Specialized Branches in Biotechnology

In glycolysis, the enzyme glyceraldehyde 3-phosphate dehydrogenase catalyzes;

#Question id: 10279

#Part-B Specialized Branches in Biotechnology

Phosphohexose isomerase is one of the enzyme of glycolysis which catalyzes Glucose 6-phosphate to Fructose 6-phosphate, enzyme bind with the hexose in their active site by opening the structure. The ring opening and closing reactions are catalyzed by an active-site present amino acid such as

#Question id: 10280

#Part-B Specialized Branches in Biotechnology

The enzyme of glycolytic pathway that forms fructose 1,6-bisphosphate is called phosphofructokinase-1 (PFK-1), which statements can’t distinguish it from a second enzyme (PFK-2) Phosphofructokinase-2.

#Question id: 10281

#Part-B Specialized Branches in Biotechnology

Gluconeogenesis is particularly important in plants such as the castor oil plant Ricinus communis and sunflower that store carbon in which form .

#Question id: 10282

#Part-B Specialized Branches in Biotechnology

In the glycolytic reaction one of the step is catalyzed by ATP-dependent phosphofructokinase is essentially irreversible, at the same step which one of the following enzyme used in gluconeogenesis, both of the enzymes are ATP dependent.

#Question id: 10287

#Part-B Specialized Branches in Biotechnology

Glyceraldehyde 3-phosphate dehydrogenase avtive site contain