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TLS Online TPP Program
#Question id: 13081
#Part-A Aptitude & General Biotechnology
Correct statements about RAPD’s
A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.
B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,
C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.
D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.
TLS Online TPP Program
#Question id: 13082
#Part-A Aptitude & General Biotechnology
Statement: AFLP shares some features of both RFLP and RAPD analyses.
Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.
II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.
TLS Online TPP Program
#Question id: 13084
#Part-A Aptitude & General Biotechnology
Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.
TLS Online TPP Program
#Question id: 13085
#Part-A Aptitude & General Biotechnology
The most common microsatellite sequence encountered in human genome is the sequence
TLS Online TPP Program
#Question id: 13153
#Part-A Aptitude & General Biotechnology
The in-radius of an equilateral triangle is of length 3 cm. Then the length of each of its medians is
TLS Online TPP Program
#Question id: 13196
#Part-A Aptitude & General Biotechnology
In The Mica Experiment Dnase I enzyme cleaves