In SDS PAGE band sharpening effect are observed, this band sharpening effect arises due to the

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#Question id: 19791

#Part-A Aptitude & General Biotechnology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

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#Question id: 19840

#Part-A Aptitude & General Biotechnology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA Polymerase	Sources
A- Tma	i) Pyrococcus furiosus
B- Deep Vent™	ii) Pyrococcus woesi
C- Tli	iii) Thermotoga maritima
D- Pfu	iv) Pyrococcus sp.
E- Pwo	v) Thermococcus litoralis

TLS Online TPP Program

#Question id: 19840

#Part-A Aptitude & General Biotechnology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA Polymerase	Sources
A- Tma	i) Pyrococcus furiosus
B- Deep Vent™	ii) Pyrococcus woesi
C- Tli	iii) Thermotoga maritima
D- Pfu	iv) Pyrococcus sp.
E- Pwo	v) Thermococcus litoralis

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#Question id: 19841

#Part-A Aptitude & General Biotechnology

Which of the following is true regarding Reverse transcriptase ?

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#Question id: 19841

#Part-A Aptitude & General Biotechnology

Which of the following is true regarding Reverse transcriptase ?

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#Question id: 19842

#Part-A Aptitude & General Biotechnology

Primer-Dimer leads to the formation of ___

a) Inter-complemetary primers

b) Hybrid 

c) Amplicons