Practice Questions

TLS Online TPP Program

#Question id: 18654

The pH of (i) stacking, (ii) resolving gel and (iii) tank buffer in SDS PAGE is respectively.

#Part-A Aptitude & General Biotechnology

(i) 8.30 (ii) 8.80 (iii) 6.80
(i) 6.80 (ii) 8.80 (iii) 8.30
(i) 8.30 (ii) 6.80 (iii) 8.80
Any of the above

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TLS Online TPP Program

#Question id: 13066

#Part-A Aptitude & General Biotechnology

Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following is a codominant marker?

TLS Online TPP Program

#Question id: 13072

#Part-A Aptitude & General Biotechnology

Inheritance pattern of RFLP and RAPD markers for genome mapping in plants;

TLS Online TPP Program

#Question id: 13073

#Part-A Aptitude & General Biotechnology

Restriction enzymes must be use in RFLP and RAPD markers for genome mapping in plants;

TLS Online TPP Program

#Question id: 13074

#Part-A Aptitude & General Biotechnology

Type of probe used in RFLP;

TLS Online TPP Program

#Question id: 13075

#Part-A Aptitude & General Biotechnology

Radioisotopes must be used in RFLP and RAPD markers

TLS Online TPP Program

#Question id: 13076

#Part-A Aptitude & General Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i. Large molecular weight genomic DNA is isolated from several strains or related species;

ii. The fragments in these digests are separated through electrophoresis

iii. These 'DNAs are then digested with a selected restriction enzyme

iv. Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v. The resulting gel lanes are transferred and fixed to a suitable solid support and

vi. The free probes are removed

vii. The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.