SDS PAGE separate molecules by size because the presence of SDS (sodium dodecyl sulphate) denatures the protein removing 2˚, 3˚ and 4˚ structures (they assume a linear chain) and the SDS coats the molecules giving them a uniform charge/mass ratio. In an experiment (SDS-PAGE) with a discontinuous buffer system following band pattern is obtained 

   

Choose the correct statement regarding this band pattern

TLS Online TPP Program

#Question id: 14259

#Part-B Specialized Branches in Biotechnology

C. roseus cells immobilized in Ca-alginate beads of diameter 0.5 mm are used for production of indole alkaloids (IA) in a fluidized-bed bioreactor. The rate limiting nutrient is glucose and no intraparticle diffusion limitations exist. Use the following data: Flow rate of the feed: Q = 1 l/h, Glucose in the feed: So = 30 g/l, Plant Cell Concentration: X = 6 g/l reac. The rate constant for IA formation: k = 5 d-1 (g/l)^-1 Ks = 0.4 g/l, Column diameter: Do = 0.15 m. Growth is negligible and Monod kinetics is valid. For 95% glucose conversion determine volume. 

TLS Online TPP Program

#Question id: 4148

#Part-A Aptitude & General Biotechnology

Which of the following is true about the sorting pathway for proteins destined for incorporation into lysosomes or the plasma membrane of eukaryotic cells?

TLS Online TPP Program

#Question id: 4309

#Part-A Aptitude & General Biotechnology

Imagine that you've isolated a yeast mutant that contains histones resistant to acetylation. What phenotype would you predict for this mutant?

TLS Online TPP Program

#Question id: 22904

#Part-B Specialized Branches in Biotechnology

B cells that produce and release large amounts of antibody are called: 

TLS Online TPP Program

#Question id: 19059

#Part-A Aptitude & General Biotechnology

Which of the following is not used for detection in GC?