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TLS Online TPP Program

#Question id: 803

A single-stranded DNA molecule contains 40 nucleotides and has the sequence 5’-(GA)20-3’. When this single strand binds to a complementary DNA strand to form a B-DNA structure,

#Part-B Specialized Branches in Biotechnology

  1. half the base-pairs will be A-G pairs and half will be C-T pairs.

  2. each base will form at least two hydrogen bonds with a base in the opposite strand.

  3. the two strands will form an antiparallel left-handed helix with 12 base pairs per turn.

  4. there will be covalent phosphodiester bonds between the two strands.

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TLS Online TPP Program

#Question id: 14260

#Part-B Specialized Branches in Biotechnology

C. roseus cells immobilized in Ca-alginate beads of diameter 0.5 mm are used for production of indole alkaloids (IA) in a fluidized-bed bioreactor. The rate limiting nutrient is glucose and no intraparticle diffusion limitations exist. Use the following data: Flow rate of the feed: Q = 1 l/h, Glucose in the feed: So = 30 g/l, Plant Cell Concentration: X = 6 g/l reac. The rate constant for IA formation: k = 5 d-1 (g/l)^-1 Ks = 0.4 g/l, Column diameter: Do = 0.15 m. Growth is negligible and Monod kinetics is valid. For 95% glucose conversion determine height of the column 
TLS Online TPP Program

#Question id: 14268

#Part-B Specialized Branches in Biotechnology

An activated-sludge waste treatment system is required to reduce the amount of BOD5 from 1000 mg/l to 20 mg/l at the exit. The sedimentation unit concentrates biomass by a factor of 3. Kinetic parameters are µm = 0.2 h-1 , Ks = 80 mg/l, kd = 0.01 h-1 , and Y M X/S = 0.5 g MLVSS/g BOD5. The flow of waste water is 10000 l/h and the size of the treatment basin is 50,000 l. What is the value of the solids residence time?
TLS Online TPP Program

#Question id: 14270

#Part-B Specialized Branches in Biotechnology

Consider a well-mixed waste treatment system for a small-scale system. The system is operated with a reactor of 1000 l and flow rate of 100 l/h. The separator concentrates biomass by a factor of 2. The recycle ratio is 0.7. The kinetic parameters are µm = 0.5 h-1 , Ks = 0.2 g/l, Y M X/S = 0.5 g/g, and kd = 0.05 h-1 . What is the exit substrate concentration?     

TLS Online TPP Program

#Question id: 14272

#Part-B Specialized Branches in Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. If qP = 0.05 g product/g cells h and P0 = 0.1 g/l, determine the product concentration in the vessel at t = 10 h
TLS Online TPP Program

#Question id: 14278

#Part-B Specialized Branches in Biotechnology

Stirrer has a power number (Np) 10. If the stirrer speed (N) is 1s^-1, its diameter D=1m and the density of the medium is 1000 kg/m^3, the ungassed power input (P) in watts is 
TLS Online TPP Program

#Question id: 14278

#Part-A Aptitude & General Biotechnology

Stirrer has a power number (Np) 10. If the stirrer speed (N) is 1s^-1, its diameter D=1m and the density of the medium is 1000 kg/m^3, the ungassed power input (P) in watts is