Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

TLS Online TPP Program

#Question id: 19888

#Part-A Aptitude & General Biotechnology

There are some typical enzyme used in the manipulation of nucleic acid;

ENZYMES	USE IN NUCLEIC ACID MANIPULATION
A) klenow	i) DNA-dependent RNA polymerase
B) T4 DNA polymerase	ii) Single-strand specific nuclease
C) Taq DNA polymerase	iii) DNA pol I lacks 5’→ 3’ exonuclease activity
D) T7 RNA polymerase	iv) Thermostable DNA polymerase used in PCR
E) S1 nuclease	v) Lack  5’→ 3’ exonuclease activity

TLS Online TPP Program

#Question id: 19929

#Part-B Specialized Branches in Biotechnology

he growth of an organism on glucose is described by the following Monod model parameters: μm = 0.5 h^-1 and Ks =0.1 g.l^-1, if the concentration of glucose in the feed is 10 g.l^-1 and the dilution rate is set to 0.4 h^-1, then the steady state concentration of glucose in the effluent will be

TLS Online TPP Program

#Question id: 19954

#Part-B Specialized Branches in Biotechnology

A continuous reactor at steady state contains 0.04 g.l^-1 of biomass and 0.02 g.l^-1 of phenol. The feed contained 0.1 g.l^-1 of phenol. The biomass yield would be

TLS Online TPP Program

#Question id: 19955

#Part-B Specialized Branches in Biotechnology

A culture system with constant environmental conditions maintained through continual provision of nutrient and removal of wastes is called __________ culture system.

TLS Online TPP Program

#Question id: 19955

#Part-A Aptitude & General Biotechnology

A culture system with constant environmental conditions maintained through continual provision of nutrient and removal of wastes is called __________ culture system.

TLS Online TPP Program

#Question id: 20043

#Part-B Specialized Branches in Biotechnology

The exponential phase may be described by the equation _______