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TLS Online TPP Program

#Question id: 14253

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

#Part-B Specialized Branches in Biotechnology
  1. 0.029m^3           
  2. 0.52m^3 
  3. 0.84m^3 
  4. 0.098m^3   
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TLS Online TPP Program

#Question id: 2967

#Part-A Aptitude & General Biotechnology

G1 is associated with which of the following cellular events?
TLS Online TPP Program

#Question id: 2968

#Part-A Aptitude & General Biotechnology

What is the name of the microtubule-organizing center found in animal cells as an identifiable structure present during all phases of the cell cycle?
TLS Online TPP Program

#Question id: 2968

#Part-B Specialized Branches in Biotechnology

What is the name of the microtubule-organizing center found in animal cells as an identifiable structure present during all phases of the cell cycle?
TLS Online TPP Program

#Question id: 2969

#Part-B Specialized Branches in Biotechnology

In the cells of many eukaryotic species, the nuclear envelope has to disappear to permit which of the following events in the cell cycle?
TLS Online TPP Program

#Question id: 2976

#Part-B Specialized Branches in Biotechnology

Match the following Regulators with its functions.

Column I

Column II

A.  Wee1 kinase

i. Degradation of phosphorylated Sic1 or p27KIP1

B. Cdc25A phosphatase

ii. Induces degradation of B-type cyclins

C. APC/CCdc20

iii. Activates vertebrate S phase CDKs

D. SCF

iv. Inhibits CDKs

Which of the following is correct?
TLS Online TPP Program

#Question id: 2982

#Part-B Specialized Branches in Biotechnology

Match the following proteins (Column I) with their functions (Column II) during cell cycle.

Column I

Column II

A. Cdc25C phosphatase

i. Activates vertebrate S phase CDKs

B. INK4

ii. Activation of Mitotic CDKs

C. Cdc25A phosphatase

iii. Binds and inhibits G1 CDKs

D. APC/CCdc20

iv. Degradation of securin