Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

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#Question id: 5693

#Part-A Aptitude & General Biotechnology

Type of nondisjunction leads to regions of tissue with different chromosome constitutions, a condition known as mosaicism is due to

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#Question id: 3613

#Part-B Specialized Branches in Biotechnology

When a single gene influences more than one trait it is called

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#Question id: 22913

#Part-B Specialized Branches in Biotechnology

Which of the following enzymes in bacteria are responsible for restricting the growth of viruses?

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#Question id: 18849

#Part-A Aptitude & General Biotechnology

Which of these techniques is often considered a suitable "polishing" step in a protein purification strategy?

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#Question id: 14529

#Part-B Specialized Branches in Biotechnology

The value of