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TLS Online TPP Program

#Question id: 3041

Which statement about gram-negative bacteria is correct?

#Part-B Specialized Branches in Biotechnology

  1.  Penicillins are the best antibiotics to use against them.

  2. They often possess an outer membrane containing toxic lipopolysaccharides.

  3. Their chromosomes are composed of DNA tightly wrapped around large amounts of histone proteins.

  4. Their cell walls are primarily composed of peptidoglycan.

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TLS Online TPP Program

#Question id: 13073

#Part-A Aptitude & General Biotechnology

Restriction enzymes must be use in RFLP and RAPD markers for genome mapping in plants;
TLS Online TPP Program

#Question id: 13074

#Part-A Aptitude & General Biotechnology

Type of probe used in RFLP;
TLS Online TPP Program

#Question id: 13075

#Part-A Aptitude & General Biotechnology

Radioisotopes must be used in RFLP and RAPD markers
TLS Online TPP Program

#Question id: 13076

#Part-A Aptitude & General Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.
TLS Online TPP Program

#Question id: 13077

#Part-A Aptitude & General Biotechnology

The DNA probes for RFLP may NOT be obtained from
TLS Online TPP Program

#Question id: 13078

#Part-A Aptitude & General Biotechnology

RFLPs have several unique advantages except this one;