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TLS Online TPP Program

#Question id: 7154

Cancer is believed to often arise from stem cells, rather than fully differentiated cells. Which of the following are consistent with this view?

#Part-B Specialized Branches in Biotechnology
  1. Stem cells are actively dividing and express few differentiated functions, so they may have fewer changes to undergo than do fully differentiated cells in becoming cancer cells.
  2. DNA replication is necessary for mutations to occur, and stem cells are replicating their DNA whereas differentiated cells are for the most part not replicating their DNA.
  3. Teratocarcinomas are tumors that arise from germ cells or embryonic stem (ES) cells, under certain circumstances, demonstrating that tumors can arise from stem cells without further mutation.
  4. All of the above are reasons that cancer cells may more readily arise from stem cells than from fully differentiated cells.
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TLS Online TPP Program

#Question id: 18775

#Part-A Aptitude & General Biotechnology

which of the following technique is a highly sensitive and quantitative procedure that utilizes radioactively labelled antigen or antibody
TLS Online TPP Program

#Question id: 18776

#Part-A Aptitude & General Biotechnology

In Western blotting, a protein mixture is separated by electrophoresis; then the protein bands are electrophoretically transferred onto nitrocellulose and identified with 
TLS Online TPP Program

#Question id: 18777

#Part-A Aptitude & General Biotechnology

which of the following technique using antibodies labelled with fluorescent molecules can be used to visualize antigen on or within cells.
TLS Online TPP Program

#Question id: 18778

#Part-A Aptitude & General Biotechnology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.
TLS Online TPP Program

#Question id: 18779

#Part-A Aptitude & General Biotechnology

Flow-cytometric analysis for the detection and measurement of subpopulations of leukocytes, including those of leukaemia, is usually performed using monoclonal antibodies. Why is this the case?
Choose whether each of the following statements is true 
a) Indirect immunofluorescence is a more sensitive technique than direct immunofluorescence
b) Most antigens induce a polyclonal response. 
c) For precipitation to occur, both antigen and antibody must be multivalent.
d) ELISA tests using chemiluminescence are more sensitive than chromogenic ones and precipitation tests are more sensitive than agglutination tests
TLS Online TPP Program

#Question id: 18780

#Part-A Aptitude & General Biotechnology

Which of the following does not participate in the formation of antigen-antibody complexes?