What is aVF in augmented unipolar limb lead?

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#Question id: 21183

#Part-A Aptitude & General Biotechnology

This PCR have mixtures of similar oligonucleotides that are used in a PCR to amplify unknown sequences of DNA, related to a known DNA sequence is

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#Question id: 18999

#Part-A Aptitude & General Biotechnology

In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure?

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#Question id: 1550

#Part-A Aptitude & General Biotechnology

IL-4 responsible for class switch recombination (CSR) into,

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#Question id: 2085

#Part-A Aptitude & General Biotechnology

Cholesterol mixes with phospholipids in a biomembrane because cholesterol molecules are

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#Question id: 14253

#Part-B Specialized Branches in Biotechnology

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics.