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TLS Online TPP Program

#Question id: 11134

Which of the following statements concerning synaptic transmission is correct?

#Part-B Specialized Branches in Biotechnology
  1. When a specific population of synaptic terminals is spread over the considerable surface of a neuron, their collective effects cannot spatially summate and lead to initiation of an action potential
  2. Even if the successive discharges of an excitatory synapse occur sufficiently close in time, they cannot temporally summate and initiate an action potential
  3. A neuron is “facilitated” when its membrane potential is moved in the less negative or depolarizing direction
  4. Even when rapidly stimulated by excitatory synaptic input for a prolonged period of time, neurons typically do not exhibit synaptic fatigue
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TLS Online TPP Program

#Question id: 13073

#Part-A Aptitude & General Biotechnology

Restriction enzymes must be use in RFLP and RAPD markers for genome mapping in plants;
TLS Online TPP Program

#Question id: 13074

#Part-A Aptitude & General Biotechnology

Type of probe used in RFLP;
TLS Online TPP Program

#Question id: 13075

#Part-A Aptitude & General Biotechnology

Radioisotopes must be used in RFLP and RAPD markers
TLS Online TPP Program

#Question id: 13076

#Part-A Aptitude & General Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.
TLS Online TPP Program

#Question id: 13077

#Part-A Aptitude & General Biotechnology

The DNA probes for RFLP may NOT be obtained from
TLS Online TPP Program

#Question id: 13078

#Part-A Aptitude & General Biotechnology

RFLPs have several unique advantages except this one;