#Question id: 4804
#Part-A Aptitude & General Biotechnology
Formation of the following pattern in an ordered octad is due to what event during meiosis? Pattern: AAaaaaaAA
#Question id: 5059
#Part-B Specialized Branches in Biotechnology
The responding tissue has already been specified and needs only an environment that allows the expression of these traits, process called as
#Question id: 1559
#Part-A Aptitude & General Biotechnology
Which of cytokine is responsible for Class Switch IgM into IgE
#Question id: 413
#Part-B Specialized Branches in Biotechnology
The hydrolysis of ATP has a large negative DG'°; nevertheless it is stable in solution due to:
#Question id: 13076
#Part-A Aptitude & General Biotechnology
Restriction fragment length polymorphism denotes that a
single restriction enzyme produces fragments of different lengths from the same
stretch of genomic DNA of different strains of a species or from different
related species. RFLPs are detected as follows:
i. Large
molecular weight genomic DNA is isolated from several strains or related
species;
ii. The
fragments in these digests are separated through electrophoresis
iii. These
'DNAs are then digested with a selected restriction enzyme
iv. Exposed
to a suitably radio-labelled appropriate DNA probe under conditions favouring
DNA: DNA hybridization
v. The
resulting gel lanes are transferred and fixed to a suitable solid support and
vi. The free
probes are removed
vii. The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.