The attraction of water to a solid phase such as a cell wall or glass surface known as

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#Question id: 19874

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Adding homopolymer tails to the 3’ end of DNA by an enzyme such as

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#Question id: 18776

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In Western blotting, a protein mixture is separated by electrophoresis; then the protein bands are electrophoretically transferred onto nitrocellulose and identified with 

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#Question id: 11861

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Four metal rods of lengths 78 cm , 104 cm , 117 cm and 169 cm are to be cut into parts of equal length. Each part must be as long as possible . What is the maximum number of pieces that can be cut ?

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#Question id: 18993

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Which of the following is not true about solvent programming which is done in high performance liquid chromatography?

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#Question id: 13058

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Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?