TLS Online TPP Program

#Question id: 13038


Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order
i. incubate with antibody-enzyme complex that binds primary antibody
ii. coat surface with antigen, block unoccupied sites with nonspecific protein
iii. add substrate, formation of colored product indicates presence of specific antigen
iv. incubate with primary antibody against specific antigen

#Part-A Aptitude & General Biotechnology
  1. i, iv, ii, iii
  2. i, iv, iii, ii
  3. ii, iv, iii, i
  4. ii, iv, i, iii