Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 16774

#Part-A Aptitude & General Biotechnology

 Some of the samples are given below. Find the median. 90, 45, 67, 34, 26, 76, 44, 55.

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#Question id: 16775

#Part-A Aptitude & General Biotechnology

If the mean and the mode are given as 35 and 30. Find the Median.

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#Question id: 16776

#Part-A Aptitude & General Biotechnology

If the Mean and Mode are 25, then find the Median.

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#Question id: 16777

#Part-A Aptitude & General Biotechnology

What is the formula for the median of Grouped data? 

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#Question id: 16778

#Part-A Aptitude & General Biotechnology

Find the Median of the following grouped data. 

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#Question id: 16779

#Part-A Aptitude & General Biotechnology

Find the Median of given Grouped data.