TLS Online TPP Program

#Question id: 13054


Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

#Part-A Aptitude & General Biotechnology
  1. The PCR reaction would not commence
  2. The PCR reaction would end after one cycle
  3. The reaction would generate a single short PCR product
  4. The reaction would yield a mixture of non-specific products
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TLS Online TPP Program

#Question id: 11284

#Part-B Specialized Branches in Biotechnology

Which of the following could qualify as a top-down control on a grassland community?

TLS Online TPP Program

#Question id: 11285

#Part-B Specialized Branches in Biotechnology

Terrestrial herbivores are animals that eat only plants and their digestive systems contain microbes that aid in the digestion of cellulose, Carnivores eat primarily animal flesh or fluids. Which of the following is correct is correct statement?

TLS Online TPP Program

#Question id: 11285

#Part-B Specialized Branches in Biotechnology

Terrestrial herbivores are animals that eat only plants and their digestive systems contain microbes that aid in the digestion of cellulose, Carnivores eat primarily animal flesh or fluids. Which of the following is correct is correct statement?

TLS Online TPP Program

#Question id: 11286

#Part-B Specialized Branches in Biotechnology

Consumption, assimilation, and production efficiencies are measures of ecological efficiency. Following some statements are correct?

(A) Consumption efficiencies for carnivores are typically higher than those of herbivores

(B) Assimilation efficiencies of herbivore are often less than consumption efficiencies

(C) Vertebrates tend to have lower production efficiencies than invertebrates

TLS Online TPP Program

#Question id: 11286

#Part-B Specialized Branches in Biotechnology

Consumption, assimilation, and production efficiencies are measures of ecological efficiency. Following some statements are correct?

(A) Consumption efficiencies for carnivores are typically higher than those of herbivores

(B) Assimilation efficiencies of herbivore are often less than consumption efficiencies

(C) Vertebrates tend to have lower production efficiencies than invertebrates

TLS Online TPP Program

#Question id: 11286

#Part-A Aptitude & General Biotechnology

Consumption, assimilation, and production efficiencies are measures of ecological efficiency. Following some statements are correct?

(A) Consumption efficiencies for carnivores are typically higher than those of herbivores

(B) Assimilation efficiencies of herbivore are often less than consumption efficiencies

(C) Vertebrates tend to have lower production efficiencies than invertebrates