Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

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#Question id: 4727

#Part-A Aptitude & General Biotechnology

if mendal had studied the seven trait using a plant with 12 chromosome instead of 14, in what manner would his interpretation have been different ?

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#Question id: 2953

#Part-A Aptitude & General Biotechnology

If a cell in the G1 phase is fused with a cell in the S phase, then

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#Question id: 749

#Part-A Aptitude & General Biotechnology

Which of the following is correct for the A form of DNA structures?

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#Question id: 5055

#Part-B Specialized Branches in Biotechnology

There are at least two components to every inductive interaction-

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#Question id: 18775

#Part-A Aptitude & General Biotechnology

which of the following technique is a highly sensitive and quantitative procedure that utilizes radioactively labelled antigen or antibody