Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

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#Question id: 18959

#Part-A Aptitude & General Biotechnology

 Vectors used for construction of genomic libraries, and their capacity is given match them correctly

A- λ EMBL series                                                                                   I - 300kb - 1,400 kb

B - Cosmids                                                                                                 II - 45 kb

C – BACs and YACs (high capacity vectors)                                             III - 20kb

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#Question id: 18960

#Part-A Aptitude & General Biotechnology

cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library

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#Question id: 18961

#Part-A Aptitude & General Biotechnology

When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called 

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#Question id: 18962

#Part-A Aptitude & General Biotechnology

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,

2. Their large size makes the handling procedures during cloning tedious and cumbersome

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.

These three problems have been resolved by deleting 

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#Question id: 18964

#Part-A Aptitude & General Biotechnology

Size of agrobacterium can be reduced by binary vector formation; these binary vectors can be formed by phenomenon of

TLS Online TPP Program

#Question id: 18978

#Part-A Aptitude & General Biotechnology

For expression vector