Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

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#Question id: 14134

#Part-B Specialized Branches in Biotechnology

PAM stands for ___

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#Question id: 14134

#Part-A Aptitude & General Biotechnology

PAM stands for ___

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#Question id: 14135

#Part-B Specialized Branches in Biotechnology

Which of the following statement is correct about PAM matrix? 

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#Question id: 14135

#Part-A Aptitude & General Biotechnology

Which of the following statement is correct about PAM matrix? 

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#Question id: 14136

#Part-B Specialized Branches in Biotechnology

Which of the following statement is incorrect about global alignment? 

(A) Global alignment is based on the Needleman–Wunsch algorithm.

(B) In Global alignment algorithm, an optimal alignment is obtained over the entire lengths of the two sequences.

(C) It is only suitable for aligning two closely related sequences that are of the same length.

(D) GAP is a web-based pairwise global alignment program.

TLS Online TPP Program

#Question id: 14136

#Part-A Aptitude & General Biotechnology

Which of the following statement is incorrect about global alignment? 

(A) Global alignment is based on the Needleman–Wunsch algorithm.

(B) In Global alignment algorithm, an optimal alignment is obtained over the entire lengths of the two sequences.

(C) It is only suitable for aligning two closely related sequences that are of the same length.

(D) GAP is a web-based pairwise global alignment program.