Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

#Question id: 467

#Part-B Specialized Branches in Biotechnology

The standard potential for electron transfer can be related to the standard free-energy change for redox reactions by using

#Question id: 532

#Part-B Specialized Branches in Biotechnology

In glycolysis, fructose 1,6-bisphosphate is converted to two products with a standard free-energy change (DG'°) of 23.8 kJ/mol.  Under what conditions (encountered in a normal cell) will the free-energy change (DG) be negative, enabling the reaction to proceed to the right?

#Question id: 533

#Part-B Specialized Branches in Biotechnology

Glucose, labeled with 14C in different carbon atoms, is added to a crude extract of a tissue rich in the enzymes of the pentose phosphate pathway.  The most rapid production of 14CO2 will occur when the glucose is labeled in:

#Question id: 544

#Part-A Aptitude & General Biotechnology

One of the enzymes involved in glycolysis, aldolase, requires Zn2+ for catalysis. Under conditions of zinc deficiency, when the enzyme may lack zinc, it would be referred to as the:

#Question id: 544

#Part-B Specialized Branches in Biotechnology

One of the enzymes involved in glycolysis, aldolase, requires Zn2+ for catalysis. Under conditions of zinc deficiency, when the enzyme may lack zinc, it would be referred to as the:

#Question id: 545

#Part-A Aptitude & General Biotechnology

Which one of the following is not among the six internationally accepted classes of enzymes?