Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 18909

#Part-A Aptitude & General Biotechnology

What is the compound X-gal?

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#Question id: 14111

#Part-A Aptitude & General Biotechnology

which of the following is a database of translated nucleic acid sequences stored in the EMBL database?

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#Question id: 1559

#Part-B Specialized Branches in Biotechnology

Which of cytokine is responsible for Class Switch IgM into IgE

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#Question id: 18731

#Part-A Aptitude & General Biotechnology

If blunt ended ligations are to be carried out. Which of the following enzymes can be used?

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#Question id: 2943

#Part-A Aptitude & General Biotechnology

Which of the following is NOT a mechanism by which G1/S cyclin-CDKs ensure progression through the cell cycle?