Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 12173

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Female with only one X chromosome is a characteristics of individual with

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#Question id: 12187

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How cells know whether sister kinetochores are properly attached to the mitotic spindle?

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#Question id: 12188

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The series of events by which APC/C promotes the separation of sister chromatids at anaphase.

A. all the kinetochores have attached to spindle microtubules and the spindle apparatus is properly assembled and oriented.

B. Cdc20 specificity factor associated with APC/C directs it to ubiquitinylate securin and mitotic cyclins. 

C. A protease known as separase cleaves the cohesin subunit Scc1 (Rad21), breaking the protein circles linking sister chromatids.

D. anaphase begins as poleward force exerted on the kinetochores moves the split sister chromatids toward opposite spindle poles.

E. Following securin degradation and a decrease in mitotic CDK activity.

Which of the following series is correct?

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#Question id: 12190

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CDK activity is modulated by the following proteins:

(a) cyclin, (b) CAK, (c) Wee1,  (d) p21. Following protein act as:

I. Inhibitory kinase

II. CKI Family of proteins

III. Activatory kinase

IV. Regulatory subunit

Which of the following is correct?

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#Question id: 12482

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The Rb protein has been called the “master brake” of the cell cycle. How the Rb protein acts as a cell cycle brake?

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#Question id: 12486

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Which of the following process occur in prometaphase?