Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

#Question id: 5721

#Part-B Specialized Branches in Biotechnology

There is paracentric inversion heterozygote ABCDEFG/ABFEDCG involved in double recombination during meiosis I within inverted region result in

A. dicentric and an acentric chromosome in meiosis I as the chiasmata get terminated.

B. appearance of deletion in both the cross over product

C. all gamete are viable

D. nonviable gametes from crossover products.

Which of the above statements are correct?

#Question id: 5724

#Part-B Specialized Branches in Biotechnology

Species I is diploid (2n  = 8) with chromosomes

AABBCCDD; related species II is diploid (2n = 8) with chromosomes MMNNOOPP. Individuals with the following sets of chromosomes represent what types of chromosome mutations?

A-   AAABBCCDD        B-   AABBCCDDMNOP

C-   AAABBCCDDD

#Question id: 5724

#Part-A Aptitude & General Biotechnology

Species I is diploid (2n  = 8) with chromosomes

AABBCCDD; related species II is diploid (2n = 8) with chromosomes MMNNOOPP. Individuals with the following sets of chromosomes represent what types of chromosome mutations?

A-   AAABBCCDD        B-   AABBCCDDMNOP

C-   AAABBCCDDD

#Question id: 5730

#Part-B Specialized Branches in Biotechnology

In a family, father is homozygous dominant (AA) for a gene A and his wife is homozygous for its ressive allele (aa) showing albino phenotype. It was surprising that their child showed the albino phenotype. Which of the following phenomenon can explain the phenotype?

#Question id: 5732

#Part-B Specialized Branches in Biotechnology

Consider a plant, such as maize, heterozygous for a paracentric inversion. The inversion is very small and a synapsed inversion loop does not occur in any of the cells. Which of the following statement is correct?

#Question id: 5733

#Part-B Specialized Branches in Biotechnology

In individuals heterozygous for a chromosome inversion, the homologous chromosomes form a loop in prophase I of meiosis.

Which of the following is effect of inversion?

A. A dicentric and an acentric chromosome in meiosis I as the chiasmata gets terminated when inversion includes centromere  

B. Appearance of deletion and duplication in both the cross over products chromosomes when centromere outside of inversion region  

C. All recombinant gamete are non-viable gametes when single crossing over within inverted region; irrespective to paracentric or pericentic inversion

D. Nonviable gametes are usually produced, resulting in a depression in observed recombination frequencies.

Which of the above statements are correct?