Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

TLS Online TPP Program

#Question id: 6558

#Part-A Aptitude & General Biotechnology

What is the next term in the following sequence 5,7,11,13,17,19,23,29,...

TLS Online TPP Program

#Question id: 5943

#Part-A Aptitude & General Biotechnology

The calendar of the year 2007 can be used again in which year ?

TLS Online TPP Program

#Question id: 14205

#Part-B Specialized Branches in Biotechnology

steady state condition is maintained in _____

TLS Online TPP Program

#Question id: 6565

#Part-A Aptitude & General Biotechnology

Find the wrong number in the series : 93, 309, 434, 498, 521, 533

TLS Online TPP Program

#Question id: 19463

#Part-A Aptitude & General Biotechnology

DIC microscopy is used for visualizing 

1. Minute details

2. Thick objects

3. Thin objects

4. Larger details

Which among the following options is correct?