Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

#Question id: 4748

#Part-A Aptitude & General Biotechnology

Eye color in Drosophila present on X chromosome, Red eye color is dominant over white eye color. There is non disjunction in Meiosis I in female and normal meiosis in male. Female is white eye color was cross with red eye male. What will be expected phenotype in their progeny?

#Question id: 4799

#Part-A Aptitude & General Biotechnology

You set up a mapping cross involving your favorite gene (YFG) in Neurospora and determine the following octad types: 4:4 arrangement 83; 2:4:2 arrangement 7; 2:2:2:2 arrangement 10. What is the distance between your gene and the centromere?

#Question id: 4800

#Part-A Aptitude & General Biotechnology

You set up a mapping cross involving two genes of interest in Saccharomyces and determine the following tetrad types: 8 nonparentalditype; 12 tetratype; 80 parental ditype. Calculate the most accurate map distance between these two genes.

#Question id: 4801

#Part-A Aptitude & General Biotechnology

You set up a mapping cross involving two genes in Aspergillus and determine the following percentages of tetrad types: parental ditype 51%, nonparentalditype 49%. What can you say about your genes?

#Question id: 4802

#Part-A Aptitude & General Biotechnology

Why is tetrad analysis useful for studying genetics?

#Question id: 4803

#Part-A Aptitude & General Biotechnology

Linked genes: