TLS Online TPP Program

#Question id: 13061


Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Arrange in the order of their increasing capacity:

#Part-A Aptitude & General Biotechnology
  1. Plasmid, BAC, Replacement vectors, Insertion vectors.
  2. Replacement, BAC, Insertion, Plasmid
  3. BAC, Insertion, Replacement, Plasmid
  4. Plasmid, Insertion, Replacement, BAC
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TLS Online TPP Program

#Question id: 10506

#Part-B Specialized Branches in Biotechnology

Plant growth promoting rhizobacteria (PGPRs) such as Pseudomonas aeruginosa, that alleviate plant abiotic or biotic stress in soil by releasing of

TLS Online TPP Program

#Question id: 10507

#Part-B Specialized Branches in Biotechnology

Rhizobacteria function as

 a) Nitrogen fixation

 b) depositing pathogens directly into the vascular system,

 c) producing antibiotics and antifungal agents for inhibiting soil pathogens

 d) herbivore sensors by sending electrical or chemical signals to surrounding cells

 Which one of the following combination is correct?

TLS Online TPP Program

#Question id: 10508

#Part-B Specialized Branches in Biotechnology

Glandular trichomes store species-specific secondary metabolites in a pocket formed between the cell wall and the cuticle. These secondary metabolites are-

TLS Online TPP Program

#Question id: 10509

#Part-B Specialized Branches in Biotechnology

A different type of mechanical obstacle to herbivory is provided by mineral crystals that are present in many plant species, such as silica crystals known as

TLS Online TPP Program

#Question id: 10510

#Part-B Specialized Branches in Biotechnology

Constitutive chemical defenses are effective against many different herbivores, including insects and mammals. Hemlock (Cicuta sp.) produces which toxins that a polyacetylene prolongs the repolarization of neuronal action potentials.

TLS Online TPP Program

#Question id: 10511

#Part-B Specialized Branches in Biotechnology

Which type of pumps inhibits digitoxin in the membranes of heart cells, leading to increased myocardial contraction?