Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Arrange in the order of their increasing capacity:

#Question id: 442

#Part-A Aptitude & General Biotechnology

Levels of ATP and ADP are partly maintained in cells by

#Question id: 16478

#Part-B Specialized Branches in Biotechnology

#Question id: 5065

#Part-B Specialized Branches in Biotechnology

Binding to the phosphorylated RTK through one of the RTK’s cytoplasmic domains, the adaptor protein also activates Ras. The activated receptor stimulates the adaptor protein to activate the:

#Question id: 2922

#Part-A Aptitude & General Biotechnology

Human genes/proteins that regulate the cell cycle are most easily isolated by:

#Question id: 5721

#Part-B Specialized Branches in Biotechnology

There is paracentric inversion heterozygote ABCDEFG/ABFEDCG involved in double recombination during meiosis I within inverted region result in

A. dicentric and an acentric chromosome in meiosis I as the chiasmata get terminated.

B. appearance of deletion in both the cross over product

C. all gamete are viable

D. nonviable gametes from crossover products.

Which of the above statements are correct?