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TLS Online TPP Program

#Question id: 13062

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

#Part-A Aptitude & General Biotechnology
  1. No bands
  2. 3 bands
  3. 5 bands
  4. 4 bands
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TLS Online TPP Program

#Question id: 10295

#Part-B Specialized Branches in Biotechnology

Reactions of the pentose phosphate pathway regeneration run six turns of this cycle, what will be predict after that?
TLS Online TPP Program

#Question id: 10296

#Part-B Specialized Branches in Biotechnology

Which of the following statements about the oxidative pentose phosphate pathway is incorrect?
TLS Online TPP Program

#Question id: 10298

#Part-B Specialized Branches in Biotechnology

Wernicke-Korsakoff syndrome is more common among people with alcoholism than in the general population, The syndrome can be exacerbated by a mutation in the gene for;
TLS Online TPP Program

#Question id: 10333

#Part-B Specialized Branches in Biotechnology

Four elements, oxygen, carbon, and hydrogen and nitrogen are the major components of most organic compounds, which of the following elements are more abundant in plants ?
TLS Online TPP Program

#Question id: 10334

#Part-B Specialized Branches in Biotechnology

N2 combines with hydrogen to form ammonia under elevated temperature (about 200°C) and high pressure (about 200 atmospheres) and in the presence of a metal catalyst (usually iron). The extreme conditions are required to overcome the high activation energy of the reaction. This nitrogen fixation reaction, called
TLS Online TPP Program

#Question id: 10335

#Part-B Specialized Branches in Biotechnology

plants can store high levels of nitrate, and they can translocate it from tissue to tissue without deleterious effect. Yet if livestock or humans consume plant material that is high in nitrate, they may suffer with,