Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

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#Question id: 11474

#Part-B Specialized Branches in Biotechnology

The blind spot in the human retina is the location that has the collected axons of ________.

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#Question id: 11861

#Part-A Aptitude & General Biotechnology

Four metal rods of lengths 78 cm , 104 cm , 117 cm and 169 cm are to be cut into parts of equal length. Each part must be as long as possible . What is the maximum number of pieces that can be cut ?

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#Question id: 388

#Part-A Aptitude & General Biotechnology

Calculate the pH of a solution prepared by mixing 2.0 mL of a strong acid solution of pH=3.0 and 3.0 mL of a strong base of pH=10.0.

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#Question id: 2279

#Part-A Aptitude & General Biotechnology

Mitochondrial enzymes for oxidative metabolism are

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#Question id: 3618

#Part-B Specialized Branches in Biotechnology

Holandric genes ar