Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

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#Question id: 377

#Part-A Aptitude & General Biotechnology

The aqueous solution with the highest pH is:

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#Question id: 380

#Part-A Aptitude & General Biotechnology

The Henderson-Hasselbalch equation:

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#Question id: 387

#Part-A Aptitude & General Biotechnology

For a weak acid with a pKa of 6.0, What would be the ratio of acid to salt at pH 5.

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#Question id: 388

#Part-A Aptitude & General Biotechnology

Calculate the pH of a solution prepared by mixing 2.0 mL of a strong acid solution of pH=3.0 and 3.0 mL of a strong base of pH=10.0.

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#Question id: 389

#Part-A Aptitude & General Biotechnology

A weak acid HA has a pKa of 5.0.  If 1.0 mol of this acid and 0.1 mol of NaOH were dissolved in one liter of water, what would the final pH be?

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#Question id: 390

#Part-B Specialized Branches in Biotechnology

What are the properties arising due to varying concentrations of solute in a given solvent, irrespective of the nature of solute with respect to the solvent?