Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

TLS Online TPP Program

#Question id: 12714

#Part-B Specialized Branches in Biotechnology

Physiological and biochemical perturbations in plants caused by fluctuations in the abiotic environment, given column I with environmental factor and with its effects in the form of primary effects  in column II and secondary effects  in column III

COLUMN I        (environmental factor )	COLUMN II            (primary effects)	COLUMN III         (secondary effects)
A) Water deficit	i) Hypoxia,  Anoxia	a) Membrane dysfunction
B) High temperature	ii) ROS production	b) Reduced cellular and metabolic activities, Leaf abscission, Ion cytotoxicity and Cavitation
C) Trace element toxicity	iii) Photo inhibition	c) Reduced respiration,     Fermentative metabolism,       Inadequate ATP production ,  ROS production and  Stomatal closure
D) Flooding and soil compaction	iv) Membrane destabilization	d) Inhibition of PSII repair
E) Chilling	v) Cell dehydration and Hydraulic resistance	e) Disruption of metabolism
F) High light intensity		f) Photosynthetic and respiratory inhibition, ROS production

Find out the correct combination of given above column;

TLS Online TPP Program

#Question id: 11142

#Part-B Specialized Branches in Biotechnology

A researcher uses the chemical inhibitor cyanide to reduce ATP production in a neuron. What would be one effect of preventing ATP production?

TLS Online TPP Program

#Question id: 19044

#Part-A Aptitude & General Biotechnology

Which of the following techniques would be most useful to identify as well as quantify the presence of a known impurity in a drug substance? 

TLS Online TPP Program

#Question id: 18920

#Part-A Aptitude & General Biotechnology

Which selection system is used in a yeast plasmid recombinant?

TLS Online TPP Program

#Question id: 14111

#Part-A Aptitude & General Biotechnology

which of the following is a database of translated nucleic acid sequences stored in the EMBL database?