Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 14797

#Part-A Aptitude & General Biotechnology

A culture of bacteria is infected in the bacteriophage at multiplicity of 0.3. The probability of a single cell infected with three phages is? 

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#Question id: 14799

#Part-B Specialized Branches in Biotechnology

In a well aerated and agitated microbial culture the supply of oxygen is equal to demand of the growing culture. The kLa for such a system will be _

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#Question id: 14801

#Part-B Specialized Branches in Biotechnology

In a chemostat operating under steady state, a bacterial culture can be grown at dilution rate higher than maximum rate by

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#Question id: 14803

#Part-B Specialized Branches in Biotechnology

The percentage SIMILARITIES and IDENTITIES, respectively, between the two peptide sequences given below will be ______% and _____%.

Peptide I :

Ala-Ala-Arg-Arg-Gln-Trp-Leu-Thr-Phe-Thr-Lys-Ile-Met-Ser-Glu

Peptide II:

Ala-Ala-Arg-Glu-Gln-Tyr-Ile-Ser-Phe-Thr-Lys-Ile-Met-Arg-Asp

TLS Online TPP Program

#Question id: 14803

#Part-A Aptitude & General Biotechnology

The percentage SIMILARITIES and IDENTITIES, respectively, between the two peptide sequences given below will be ______% and _____%.

Peptide I :

Ala-Ala-Arg-Arg-Gln-Trp-Leu-Thr-Phe-Thr-Lys-Ile-Met-Ser-Glu

Peptide II:

Ala-Ala-Arg-Glu-Gln-Tyr-Ile-Ser-Phe-Thr-Lys-Ile-Met-Arg-Asp

TLS Online TPP Program

#Question id: 14804

#Part-B Specialized Branches in Biotechnology

Calculate the percentage sequence identity for the pairwise alignment given below: 

                       H E L L O _

                       Y E L L O W