Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

#Question id: 5378

#Part-A Aptitude & General Biotechnology

Out of 10 persons , 9 persons spent Rs 40 each of their meals .The 10th one spent Rs 18 more than the average of expenditure of all the 10 . the total money spent by all of them is 

#Question id: 5379

#Part-A Aptitude & General Biotechnology

The average weight of three brothers is 68 kg . If their weights are in the ratio 3:4:5, the weight of the heaviest of the three brothers is.

#Question id: 5380

#Part-A Aptitude & General Biotechnology

Out of three numbers the second number is twice the first and also one third of third number.If the average of three numbers is 54. Find the second number.

#Question id: 5381

#Part-A Aptitude & General Biotechnology

A man travels from destination A and B by car to an average speed of 48 km/h and returns on his bike with an average speed of 16 km/h .Find his average speed for the entire journey

#Question id: 5382

#Part-A Aptitude & General Biotechnology

The average of weight of 5 persons is decreased by 2 kg when one of the men whose weight is 50kg is replaced by a new man. The weight of the new man is.

#Question id: 5384

#Part-A Aptitude & General Biotechnology

The average of 100 numbers is 50. It is found that while calculating the average two numbers namely 81 and 66 were wrongly read as 18 and 6 the correct average is.