Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Agglutination of antigens by utilising specific antibodies can only occur, if

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#Question id: 21380

#Part-B Specialized Branches in Biotechnology

Sum of the powers of the concentration terms in the rate equation is called the __________ of the reaction.

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#Question id: 21726

#Part-B Specialized Branches in Biotechnology

For a first order chemical reaction, the rate constant

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#Question id: 21727

#Part-B Specialized Branches in Biotechnology

In the reaction H2O2(aq) → H2O(l) + ½ O2(g), the initial concentration of H2O2 is 0.2546 M, and the initial rate of reaction is 9.32×10^–4 M s^–1. What will be [H2O2] at t = 35 s?

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#Question id: 21728

#Part-B Specialized Branches in Biotechnology

In the reaction A → products, we find that when [A] has fallen to half of its initial value, the reaction proceeds at the same rate as its initial rate. Is the reaction zero order, first order, or second order?

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#Question id: 21729

#Part-B Specialized Branches in Biotechnology

In the reaction, A → products, with the initial concentration [A]0 = 1.512 M, [A] is found to be 1.496 M at t = 30 s. With the initial concentration [A]0 = 2.584 M, [A] is found to be 2.552 M at t = 1 min. What is the order of this reaction?

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#Question id: 21731

#Part-B Specialized Branches in Biotechnology

The smog constituent peroxyacetyl nitrate (PAN) dissociates into peroxyacetyl radicals and NO2(g) in a first order reaction with a half-life of 32 min. 

If the initial concentration of PAN in an air sample is 2.7×10¹⁵ molecules/L, what will be the concentration 2.24 h later?