Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Agglutination of antigens by utilising specific antibodies can only occur, if

#Question id: 3216

#Part-A Aptitude & General Biotechnology

The frequency of galactosemia is approximately 1 in 40,000 live births. The frequency of the carrier state can be calculated as

#Question id: 3250

#Part-A Aptitude & General Biotechnology

Consider a diploid population at Hardy-Weinberg equilibrium. For a locus with two alleles, the frequency of the A1A1, A1A2 and A2A2 genotype is 0.01, 0.18 and 0.81) Which of the following statement is correct?

#Question id: 3251

#Part-A Aptitude & General Biotechnology

Looking at 3 allele per locus and following Hardy Weinberg equilibrium what will be the expected heterozygosity if q1=0.5, q2=0.3, q3=0.2

#Question id: 3252

#Part-A Aptitude & General Biotechnology

 The unaffected brother of a cystic fibrosis affected girl is referred for genetic counseling. Assume that the population is in Hardy-Weinberg equilibrium. Which of the following is correct?  (Cystic fibrosis is an autosomes recessive inheritance with an incidence of 1 in 2500).

#Question id: 3253

#Part-A Aptitude & General Biotechnology

In a population 64% individuals of 1000 population are affected with autosomes dominant diseased. How many individuals are expected carry at least one dominant allele?

#Question id: 3254

#Part-A Aptitude & General Biotechnology

Consider a single locus with 2 alleles which are at Hardy-Weinberg equilibrium. If the frequency of the heterozygous genotypes is 0.42, what is the frequency of one homozygote in the population?