Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following is a codominant marker?

#Question id: 10350

#Part-B Specialized Branches in Biotechnology

Ammonium can be assimilated via an alternative pathway rather than primary pathway which involves the sequential actions of glutamine synthetase(GS) and glutamate synthase(GOGAT), these alternative pathway catalyzes by enzyme known as,

#Question id: 10352

#Part-B Specialized Branches in Biotechnology

which of the following amino acid that participates in the malate–aspartate shuttle to transfer reducing equivalents from the mitochondrion and chloroplast into the cytosol?

#Question id: 10353

#Part-B Specialized Branches in Biotechnology

Aminotransferases are found in multiple organelles such as cytoplasm, chloroplasts, mitochondria, glyoxysomes, and peroxisomes, these aminotransferases one of its organelles may have a significant role in amino acid biosynthesis such as;

#Question id: 10357

#Part-B Specialized Branches in Biotechnology

Why the glutamine and glutamate will not be transported while  asparagine will prefer to be transported?

#Question id: 10358

#Part-B Specialized Branches in Biotechnology

Which of the following essential micronutrients is associated with nitrate reductase enzyme found in higher plants?

#Question id: 10359

#Part-B Specialized Branches in Biotechnology

Nitrate reductases of higher plants are composed of two identical subunits, each containing three prosthetic groups. Which of the following is not the prosthetic group of nitrate reductase;