Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following is a codominant marker?

#Question id: 789

#Part-B Specialized Branches in Biotechnology

Presence of large numbers of modified specially methylated nucleotides in tRNA contribute in

#Question id: 790

#Part-B Specialized Branches in Biotechnology

Presence of a series of 3 G-C pairs in the anticodon arm of initiator fmet tRNA is essential for

#Question id: 792

#Part-B Specialized Branches in Biotechnology

Which arm of the tRNA can act as a structural recognition feature?

#Question id: 794

#Part-B Specialized Branches in Biotechnology

A single-stranded DNA molecule contains 40 nucleotides with equal amounts of A, C, G, and T. This DNA strand can combine with a complementary DNA strand to form a double-stranded DNA molecule. Which structural feature is found in the single-stranded DNA molecule?

#Question id: 795

#Part-B Specialized Branches in Biotechnology

A single-stranded DNA molecule contains 40 nucleotides and has the sequence 5’-(GA)20-3’. When this single strand binds to a complementary DNA strand,

#Question id: 797

#Part-B Specialized Branches in Biotechnology

With respect to microRNAs, which, if any, of the following statements, is FALSE?)

A. MicroRNA genes often occur in gene clusters within introns of protein-coding genes and are usually transcribed by RNA polymerase II.

B. Like an mRNA a miRNA is initially produced as a larger precursor RNA that, like the great majority of mRNAs, usually has a 5’ cap and a poly(A) tail.

C. MicroRNAs are produced by germ-line cells only.

D. A miRNA is initially produced as a duplex RNA but in order to work it needs to be converted into a single-strand RNA.

E. MicroRNAs usually work as transcription factors.