Statement: AFLP shares some features of both RFLP and RAPD analyses.

Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.

#Question id: 794

#Part-B Specialized Branches in Biotechnology

A single-stranded DNA molecule contains 40 nucleotides with equal amounts of A, C, G, and T. This DNA strand can combine with a complementary DNA strand to form a double-stranded DNA molecule. Which structural feature is found in the single-stranded DNA molecule?

#Question id: 795

#Part-B Specialized Branches in Biotechnology

A single-stranded DNA molecule contains 40 nucleotides and has the sequence 5’-(GA)20-3’. When this single strand binds to a complementary DNA strand,

#Question id: 797

#Part-B Specialized Branches in Biotechnology

With respect to microRNAs, which, if any, of the following statements, is FALSE?)

A. MicroRNA genes often occur in gene clusters within introns of protein-coding genes and are usually transcribed by RNA polymerase II.

B. Like an mRNA a miRNA is initially produced as a larger precursor RNA that, like the great majority of mRNAs, usually has a 5’ cap and a poly(A) tail.

C. MicroRNAs are produced by germ-line cells only.

D. A miRNA is initially produced as a duplex RNA but in order to work it needs to be converted into a single-strand RNA.

E. MicroRNAs usually work as transcription factors.

#Question id: 799

#Part-B Specialized Branches in Biotechnology

The two cleavage reactions required to generate the miRNA from these primary transcripts are mediated by two distinct RNases. A characteristic of both these enzymes is that they recognize and cleave RNAs on the basis of the structure of their substrates rather than their specific sequence. Which of the following statement is INCORRECT regarding the cleavage reactions that produces a mature RNA?

#Question id: 801

#Part-B Specialized Branches in Biotechnology

Identify the type of DNA from the structures given below and match the correct form with its characteristics described.

#Question id: 803

#Part-B Specialized Branches in Biotechnology

A single-stranded DNA molecule contains 40 nucleotides and has the sequence 5’-(GA)20-3’. When this single strand binds to a complementary DNA strand to form a B-DNA structure,