Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.

Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.

II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

TLS Online TPP Program

#Question id: 19457

#Part-A Aptitude & General Biotechnology

Which microscopy can be used for viewing live cells and unstained tissues?

1. Fluorescence microscopy

2. Phase contrast microscopy

3. Dark field microscopy

4. Differential interference microscopy

5. Confocal microscopy

Which of the above combinations of microscopy is correct?

TLS Online TPP Program

#Question id: 19457

#Part-B Specialized Branches in Biotechnology

Which microscopy can be used for viewing live cells and unstained tissues?

1. Fluorescence microscopy

2. Phase contrast microscopy

3. Dark field microscopy

4. Differential interference microscopy

5. Confocal microscopy

Which of the above combinations of microscopy is correct?

TLS Online TPP Program

#Question id: 19458

#Part-A Aptitude & General Biotechnology

How phase contrast and DIC microscopy are used to image live cells and unstained tissues?

TLS Online TPP Program

#Question id: 19458

#Part-B Specialized Branches in Biotechnology

How phase contrast and DIC microscopy are used to image live cells and unstained tissues?

TLS Online TPP Program

#Question id: 19459

#Part-A Aptitude & General Biotechnology

Phase-contrast microscopy depends on the refractive index of the region of specimen and generates image in which we see a pattern of 

TLS Online TPP Program

#Question id: 19460

#Part-A Aptitude & General Biotechnology

In phase contrast unobstructed light passes through a region of________ that both transmits only a small percentage of the light and changes its phase slightly.