The series of events by which APC/C promotes the separation of sister chromatids at anaphase.

A. all the kinetochores have attached to spindle microtubules and the spindle apparatus is properly assembled and oriented.

B. Cdc20 specificity factor associated with APC/C directs it to ubiquitinylate securin and mitotic cyclins. 

C. A protease known as separase cleaves the cohesin subunit Scc1 (Rad21), breaking the protein circles linking sister chromatids.

D. anaphase begins as poleward force exerted on the kinetochores moves the split sister chromatids toward opposite spindle poles.

E. Following securin degradation and a decrease in mitotic CDK activity.

Which of the following series is correct?

#Question id: 13075

#Part-A Aptitude & General Biotechnology

#Question id: 13076

#Part-A Aptitude & General Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 13077

#Part-A Aptitude & General Biotechnology

#Question id: 13078

#Part-A Aptitude & General Biotechnology

#Question id: 13079

#Part-A Aptitude & General Biotechnology

#Question id: 13080

#Part-A Aptitude & General Biotechnology