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TLS Online TPP Program
#Question id: 13054
#Part-A Aptitude & General Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?
TLS Online TPP Program
#Question id: 19454
#Part-B Specialized Branches in Biotechnology
Resolving power has the ability to distinguish between
TLS Online TPP Program
#Question id: 19174
#Part-A Aptitude & General Biotechnology
Which of the following cannot be used as adsorbent in Column adsorption chromatography?
TLS Online TPP Program
#Question id: 747
#Part-A Aptitude & General Biotechnology
In nucleic acids, the bases are covalently attached to the 1-position of a pentose sugar ring, to form a:
TLS Online TPP Program
#Question id: 19055
#Part-A Aptitude & General Biotechnology
In quantitative T.L.C radioactive material can be studied by