For more complex reactions immobilized______will be used

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#Question id: 13064

#Part-A Aptitude & General Biotechnology

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 3679

#Part-A Aptitude & General Biotechnology

A mutation in an E. coli cell becomes active half-way through a round of replication. The cell completes the round of replication normally but then cannot start a second round. Which protein could be mutated?

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#Question id: 10892

#Part-B Specialized Branches in Biotechnology

Considering environment and ecosystem, identify the INCORRECT statement

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#Question id: 2956

#Part-A Aptitude & General Biotechnology

The product of the cdc2 gene is

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#Question id: 841

#Part-B Specialized Branches in Biotechnology

In E.coli chapronin activity contains  two subunit GroEL and GroES, binding the protein with the GroEL changes the conformations and hydrolysis of ATP would be  takes place. In which conformation of GroEL bound protein to get converted into folded?