KKXX sorting signal,

binds to a complex of the COPI α and β subunits

TLS Online TPP Program

#Id: 8853

#Section 3: Genetics, Cellular and Molecular Biology

Cohesins 

The protein ring complexes that establish linkages between sister chromatids

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#Id: 8854

#Section 3: Genetics, Cellular and Molecular Biology

Cohesins establish linkages between the replicated DNA molecules, which are essential for their accurate segregation later in the cell cycle. This linking mechanism is coupled to DNA replication.

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#Id: 8855

#Section 3: Genetics, Cellular and Molecular Biology

Cohesins are composed of four subunits: 

Smc1, Smc3, Scc1, and Scc3

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#Id: 8856

#Section 3: Genetics, Cellular and Molecular Biology

Smc1 and Smc3 are members of the structural

maintenance of chromosomes (SMC) protein family, characterized by globular domains at the N- & C-termini connected by a long α helix, bind and hydrolyze ATP.

TLS Online TPP Program

#Id: 8857

#Section 3: Genetics, Cellular and Molecular Biology

The ATPase domains interact with the proteins

Scc1 and Scc3, which together form a cohesin ring structure which embrace one or both copies of the replicated DNA.

TLS Online TPP Program

#Id: 8858

#Section 3: Genetics, Cellular and Molecular Biology

Cohesin-mediated cohesion between sister chromatids is established by a two-step process:

1.Loading and unloading of cohesins

2. Stabilization of cohesins