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TLS Online TPP Program

#Question id: 14159

Which of the following statement is not a benefit of FASTA over BLAST?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. FASTA scans smaller window sizes.
  2. FASTA gives more sensitive results than BLAST
  3. FASTA is usually slower than BLAST
  4. FASTA identifies identical matching words using the hashing procedure while BLAST uses a substitution matrix to find matching words.
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TLS Online TPP Program

#Question id: 14220

#Section 5: Bioprocess Engineering and Process Biotechnology

A value of kLa = 30 h^-1 has been determined for a fermenter at its maximum practical agitator rotational speed and with air being sparged at 0.5 l gas/l reactor volume-min. E. coli with a qO2 of 10 mmol O2/g-dry wt-h are to be cultured. The critical dissolved oxygen concentration is 0.2 mg/l. The solubility of oxygen from air in the fermentation broth is 7.3 mg/l at 30⁰C.
What maximum concentration of E. coli can be sustained in this fermenter under aerobic conditions?
TLS Online TPP Program

#Question id: 13023

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following enzymes are required for end labelling?
TLS Online TPP Program

#Question id: 14621

#Section 5: Bioprocess Engineering and Process Biotechnology

a chromatographic separation of a two component samples on 50 cm column gave the retention times for the solutes A and B as 2.5 and 3.1 minutes with base widths of the two chromatographic peaks being 0.24 and 0.3 min respectively. Calculate plate height 
TLS Online TPP Program

#Question id: 14327

#Section 5: Bioprocess Engineering and Process Biotechnology

Bakers' yeast is produced in a 50 000-litre fermenter under aerobic conditions. The carbon substrate is sucrose; ammonia is provided as nitrogen source. The average biomass composition is CH1.8300.55N0.17 with 5% ash. Under efficient growth conditions, biomass is the only major product; the biomass yield from sucrose is 0.5 g g-I. If the specific growth-rate is 0.45 h-l, estimate the rate of heat removal required to maintain constant temperature in the fermenter when the yeast concentration is 10 g l- 1     _______________________.10^6
TLS Online TPP Program

#Question id: 14743

#Section 5: Bioprocess Engineering and Process Biotechnology

for the fermenter with the given data. 
Air is sparged with 0.4 vvm, it is equipped with two sets of impellers and a flat-blade turbine with four baffles. calculate power, P