Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C_4.4H_7.30 _1.2N_0.86 . These bacteria are grown under aerobic conditions with hexadecane (C₁₆H₃₄) as substrate. The reaction equation describing growth is:

                                  

  Assuming 100% conversion, what is the yield of cells from hexadecane in g g^-l?   

TLS Online TPP Program

#Question id: 14742

#Section 5: Bioprocess Engineering and Process Biotechnology

Gel chromatography is used for hormone separation. A pilot-plant scale gel chromatography column packed with sephacryl resin is used to separate two hormones, A and B. The column is 5 cm in diameter and 30 cm in height: V, = 1.9x 10-⁴m³, Wr = 3x10^-3m³ /kg dry sephacryl, ps =1.25 x 10³ kg/m³ . The partition coefficients are k_pA =0.38 and k_pB= 0.15. If the eluting flow rate is 0.7 I/h, what is the retention time for each hormone? 

TLS Online TPP Program

#Question id: 14743

#Section 5: Bioprocess Engineering and Process Biotechnology

for the fermenter with the given data. 

Air is sparged with 0.4 vvm, it is equipped with two sets of impellers and a flat-blade turbine with four baffles. calculate power, P 

TLS Online TPP Program

#Question id: 14744

#Section 5: Bioprocess Engineering and Process Biotechnology

For production of an enzyme used for synthesis of a sun protection lotion, the required kinetic data and constants are: 

vmax = 2.3 mmol.m^-3 s^-1 Km = 8.9 mmol.^-1 So = 12 mmol1- 

Find the reaction time for 95% conversion of raw material 

TLS Online TPP Program

#Question id: 14745

#Section 5: Bioprocess Engineering and Process Biotechnology

heat exchanger, one transfer unit means 

TLS Online TPP Program

#Question id: 14746

#Section 5: Bioprocess Engineering and Process Biotechnology

During cultivation of microorganism in a fermenter, various parameters are controlled by appropriate mechanism, match the following 

TLS Online TPP Program

#Question id: 14747

#Section 5: Bioprocess Engineering and Process Biotechnology

Mechanism of separation of contaminants present in air by fibrous media are.