TLS Online TPP Program

#Question id: 14169


Unsweetened condensed milk is a shelf-stable canned milk product. If 100kg of fresh milk, containing 87% water and the rest milk solids, is condensed using an evaporator to remove 60% of the water from the fresh milk, calculate:

 The mass of water evaporated


#Section 5: Bioprocess Engineering and Process Biotechnology
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TLS Online TPP Program

#Question id: 634

#Section 2: General Biology

An enzyme has a Vmax of 50 micromol product formed (minute x mg protein)-1 and a Km of 10 microM for the substrate. When a reaction mixture contains the enzyme and 5 microM substrate, which of the following percentages of the maximum velocity will be closest to the initial reaction rate?

TLS Online TPP Program

#Question id: 635

#Section 2: General Biology

The enymatic rate constant (kcat/Km) of orotidine 5ʹ-phosphate decarboxylase is 6 x 107 M-1s-1 and the nonenzymatic rate constant (kn) is 3 x 10-16 s-1.  What is the value of the enzymeʹs catalytic proficiency?

TLS Online TPP Program

#Question id: 636

#Section 2: General Biology

DNA polymerase contains a lysine residue that is important for binding to DNA. Mutations were found that converted this lysine to either glutamate, glycine, valine, or arginine. Which mutations would be predicted to be the most and least harmful to the ability of the enzyme to bind DNA?

Most

Least

(A)

Valine

Aspartate

(B)

Glycine

Arginine

(C)

Arginine

Glycine

(D)

Glutamate

Arginine

TLS Online TPP Program

#Question id: 638

#Section 2: General Biology

Match the following class of enzymes with them

EnzymeClass of enzyme
A. Hexokinase1
B. Phosphoglucoisomerase2
C. Glyceraldehyde 3-phosphate dehydrogenase3
D. Phosphoglycerate mutase4
E. Enolase5
F. Aldolase

TLS Online TPP Program

#Question id: 639

#Section 2: General Biology

Match the coenzymes in column I enzymes to class of enzymes in column II

EnzymeReaction Type
A. Phosphofructokinase1. Redox reaction
B. Enolase2. Phosphoryl shift
C. Phosphoglycerate mutase3. Dehydration
D. Glyceraldehyde 3 Phosphate dehydrogenase4. Phosphoryl transfer

TLS Online TPP Program

#Question id: 640

#Section 2: General Biology

The data in the table above were collected for an enzyme-catalyzed reaction. The Km for this enzyme is approximately