TLS Online TPP Program

#Question id: 14175


The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction

Determine the biomass yield coefficient YX/S? 


#Section 5: Bioprocess Engineering and Process Biotechnology
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TLS Online TPP Program

#Question id: 607

#Section 2: General Biology

In E. coli ________ is an activator and ________ is a negative allosteric modulator of the enzyme phosphofructokinase-1. 

TLS Online TPP Program

#Question id: 14194

#Section 5: Bioprocess Engineering and Process Biotechnology

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the culture volume at t = 10 h

TLS Online TPP Program

#Question id: 14930

#Section 5: Bioprocess Engineering and Process Biotechnology

E. coli grows from 0.10 to 0.50 kg dry cell m-3 in 1 h. Assuming exponential growth during this period, evaluate the specific growth rate 

TLS Online TPP Program

#Question id: 58

#Section 2: General Biology

A covalent chemical bond is one in which ________.

TLS Online TPP Program

#Question id: 27360

#Section 5: Bioprocess Engineering and Process Biotechnology

The same culture (with the m and K, values as computed above) is cultivated in a 10 L chemostat being operated with a 50 mL min-1 sterile feed containing 50 g L-1 of substrate. Assuming an overall yield coefficient of 0.3 (g- DCW) (g- substrate), the respective values of the outlet biomass and substrate concentrations are