Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V₀ = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S₀ = 300 g/l and X₀ = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h^-1 , K_S = 0.5 g/l, and Y_X/S = 0.3 g dw/g glucose. Determine the culture volume at t = 10 h

#Question id: 14199

#Section 5: Bioprocess Engineering and Process Biotechnology

#Question id: 14200

#Section 5: Bioprocess Engineering and Process Biotechnology

A fluidized-bed, immobilized-cell bioreactor is used for the conversion of glucose to ethanol by Z. mobilis cells immobilized in k-carrageenan gel beads. The dimensions of the bed are 10 cm (diameter) by 200 cm. Since the reactor is fed from the bottom of the column and because of CO₂ gas evolution, substrate and cell concentrations decrease with the height of the column. The average cell concentration at the bottom of the column is X₀ = 45 g/l, and the average cell concentration decreases with the column height according to the following equation:

where Z is the column height (cm). The specific rate of substrate consumption is q_S = 2 g S/g cells . h. The feed flow rate and glucose concentration in the feed are 5 l/h and 160 g glucose/l, respectively.

Determine the ethanol concentration in the effluent (g/l . h) if YP/S = 0.48 g ethanol/g glucose

#Question id: 14201

#Section 5: Bioprocess Engineering and Process Biotechnology

A fluidized-bed, immobilized-cell bioreactor is used for the conversion of glucose to ethanol by Z. mobilis cells immobilized in k-carrageenan gel beads. The dimensions of the bed are 10 cm (diameter) by 200 cm. Since the reactor is fed from the bottom of the column and because of CO₂ gas evolution, substrate and cell concentrations decrease with the height of the column. The average cell concentration at the bottom of the column is X₀ = 45 g/l, and the average cell concentration decreases with the column height according to the following equation:

where Z is the column height (cm). The specific rate of substrate consumption is q_S = 2 g S/g cells . h. The feed flow rate and glucose concentration in the feed are 5 l/h and 160 g glucose/l, respectively.

Determine the ethanol productivity (g/l . h) if YP/S = 0.48 g ethanol/g glucose.

_______________

#Question id: 14202

#Section 5: Bioprocess Engineering and Process Biotechnology

In a fluidized-bed biofilm reactor, cells are attached on spherical plastic particles to form biofilms of average thickness L = 0.5 mm. The bed is used to remove carbon compounds from a waste-water stream. The feed flow rate and concentration of total fermentable carbon compounds in the feed are F = 2 l/h and S = 2000 mg/l. The diameter of the column is 10 cm. The kinetic constants of the microbial population are r_m = 50 mg S/cm³. h and K_S = 25 mg S/cm³ . The specific surface area of the biofilm in the reactor is 2.5 cm² /cm³ . Assuming first-order reaction kinetics and an average effectiveness factor of n = 0.7 throughout the column, determine the required height of the column for effluent total carbon concentration of S_0i = 100 mg/l.

#Question id: 14203

#Section 5: Bioprocess Engineering and Process Biotechnology

#Question id: 14204

#Section 5: Bioprocess Engineering and Process Biotechnology