Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V₀ = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S₀ = 300 g/l and X₀ = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h^-1 , K_S = 0.5 g/l, and Y_X/S = 0.3 g dw/g glucose. Determine the culture volume at t = 10 h

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#Question id: 16074

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the items in Group 1 with an appropriate description in Group 2 

Group 1                                                    Group 2 

P. PSI-BLAST                                    1. ungapped, aligned motif 

Q. CLUSTALW                                 2. Profiles

R. BLOCK                                         3. Heuristic method

S. FASTA                                           4. Multiple sequence alignment

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#Question id: 27318

#Section 5: Bioprocess Engineering and Process Biotechnology

The specific productivity (qp) of an enzyme production is fitted linearly with specific growth rate (μ) of a fungal organism according to the equation qp = α.μ + β. The estimated values of constants α and β are 0.0006 and 25 respectively. The enzyme production kinetics is

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#Question id: 3523

#Section 3: Genetics, Cellular and Molecular Biology

If the number of heterozygous pairs involved in a particular cross is three, the phenotype ratio obtained in their F2 generation will be-

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#Question id: 3356

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following above way not cause sampling error in succeed generation?

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#Question id: 4347

#Section 3: Genetics, Cellular and Molecular Biology

According to the lac operon model proposed by Jacob and Monod, what is predicted to occur if the operator is removed from the operon?