Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V₀ = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S₀ = 300 g/l and X₀ = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h^-1 , K_S = 0.5 g/l, and Y_X/S = 0.3 g dw/g glucose. Determine the concentration of glucose at t = 10 h at quasi-steady state

#Question id: 14321

#Section 5: Bioprocess Engineering and Process Biotechnology

Both Saccharomyces cerevisiae yeast and Zymomonas mobilis bacteria produce ethanol from glucose under anaerobic conditions without external electron acceptors. The biomass yield from glucose is 0.11 g g-1 for yeast and 0.05 g g-I for Z. mobilis. In both cases the nitrogen source is NH 3. Both cell compositions are represented by the formula CH 1.800.5N0.2. Compare actual yield with theoretical maximum for Zymomonas mobilis bacteria ?

#Question id: 14322

#Section 5: Bioprocess Engineering and Process Biotechnology

Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CH_I.550_0.31N_0.25. The yield ofbiomass from glucose is measured at 0.48 g g^-1; the yield of recombinant protein from glucose is about 20% that for cells. How much ammonia is required per gmol glucose?

#Question id: 14323

#Section 5: Bioprocess Engineering and Process Biotechnology

Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CHI.5500.31N0.25. The yield ofbiomass from glucose is measured at 0.48 g g-1; the yield of recombinant protein from glucose is about 20% that for cells. What is the oxygen demand per gmol glucose?

#Question id: 14324

#Section 5: Bioprocess Engineering and Process Biotechnology

Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CHI.5500.31N0.25. The yield of biomass from glucose is measured at 0.48 g g^-1; the yield of recombinant protein from glucose is about 20% that for cells. If the biomass yield remains at 0.48 g g^{- l}, how much change in oxygen requirement for wild-type E. coli unable to synthesise recombinant protein?

#Question id: 14325

#Section 5: Bioprocess Engineering and Process Biotechnology

Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CHI.5500.31N0.25. The yield ofbiomass from glucose is measured at 0.48 g g-1; the yield of recombinant protein from glucose is about 20% that for cells. If the biomass yield remains at 0.48 g g- l, how much change in ammonia requirement for wild-type E. coliunable to synthesise recombinant protein?

#Question id: 14326

#Section 5: Bioprocess Engineering and Process Biotechnology

The chemical reaction equation for conversion of ethanol (C₂H₆0) to acetic acid (C₂H₄0₂) is:

Acetic acid is produced from ethanol during growth of Acetobacter aceti, which has the composition CH_1.80_0.5N_0.2. Biomass yield from substrate is 0.14 g g^{- 1}; product yield from substrate is 0.92 g g^-1. Ammonia is used as nitrogen source. How much gmol of oxygen is demanded for acetic acid production?