TLS Online TPP Program

#Question id: 14221


E. coli have a maximum respiration rate, qO2max, of about 240-mg O2/g-dry wt-h. It is desired to achieve a cell mass of 20 g dry wt/l. The kLa is 120 h-1 in a 1000-l reactor (800 l working volume). A gas stream enriched in oxygen is used (i.e., 80% O2) which gives a value of C* = 28 mg/L. If oxygen becomes limiting, growth and respiration slow; for example,

                                     
   where CL is the dissolved oxygen concentration in the fermenter. What is CL when the cell mass is  at 20 g/l?

#Section 5: Bioprocess Engineering and Process Biotechnology
  1. 0.44mg/l     
  2. 0.25mg/l   
  3. 0.84mg/l           
  4. 0.64mg/l