A fermenter is maintained at 35⁰C by water circulating at a rate of 0.5 kg s^-1 in a cooling coil inside the vessel. The inlet and outlet temperatures of the water are 8⁰C and 15⁰C respectively. The length of the cooling coil is increased by 50%. In order to maintain the same fermentation temperature, the rate of heat removal must be kept the same. Determine the new cooling-water flow rate and outlet temperature by carrying out the following calculations. The heat capacity of the cooling water can be taken as 4.18 kJ kg^- 1 o C^- 1. Determine the mean temperature difference with the original coil.

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#Question id: 18778

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.

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#Question id: 14253

#Section 5: Bioprocess Engineering and Process Biotechnology

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

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#Question id: 3582

#Section 2: General Biology

Different genetic cross regarding two independently assorting non allelic gene with complete dominance and their phenotypic ratio showed in a following is correc

	Genetic cross		Phenotype ratio
I	AABb X AaBb	A	1:1
II	AaBb X  aaBB	B	3:3:1:1
III	Aabb X aaBb	C	6:2
IV	AaBb X Aabb	D	1:1:1:1

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#Question id: 14609

#Section 5: Bioprocess Engineering and Process Biotechnology

According to Hofmeister series, which anions series are correct in decreasing order according to their effectiveness in causing precipitation?

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#Question id: 15866

#Section 6: Plant, Animal and Microbial Biotechnology

Tissues and cells cultured in a liquid medium produce a single cells and cells clumps of few to many cells; these are called;