Nutrient medium is to be heated from 10⁰C to 28⁰C is a single-pass countercurrent shell-and-tube heat exchanger before being pumped into a fed-batch fermenter. Medium passes through the tubes of the exchanger; the shell-side fluid is water which enters with flow rate 3 x 10^4 kg h^- 1 and temperature 60⁰C pre-heated medium is required at a rate of 50 m^3 h^-1. The density, viscosity and heat capacity of the medium are the same as water; the thermal conductivity of the medium is 0.54 Wm^- 1 ⁰C^ - 1. It is proposed to use 30 steel tubes with inner diameter 5 cm; the tubes will be arranged in line. The pipe wall is 5-mm thick; the thermal conductivity of the metal is 50 Wm^- 1 ⁰C^ - 1. The maximum linear shell-side fluid velocity is estimated as 0.15 m s^-1. What is the rate of heat transfer?

TLS Online TPP Program

#Question id: 14260

#Section 5: Bioprocess Engineering and Process Biotechnology

C. roseus cells immobilized in Ca-alginate beads of diameter 0.5 mm are used for production of indole alkaloids (IA) in a fluidized-bed bioreactor. The rate limiting nutrient is glucose and no intraparticle diffusion limitations exist. Use the following data: Flow rate of the feed: Q = 1 l/h, Glucose in the feed: So = 30 g/l, Plant Cell Concentration: X = 6 g/l reac. The rate constant for IA formation: k = 5 d-1 (g/l)^-1 Ks = 0.4 g/l, Column diameter: Do = 0.15 m. Growth is negligible and Monod kinetics is valid. For 95% glucose conversion determine height of the column 

TLS Online TPP Program

#Question id: 14261

#Section 5: Bioprocess Engineering and Process Biotechnology

C. roseus cells immobilized in Ca-alginate beads of diameter 0.5 mm are used for production of indole alkaloids (IA) in a fluidized-bed bioreactor. The rate limiting nutrient is glucose and no intraparticle diffusion limitations exist. Use the following data: Flow rate of the feed: Q = 1 l/h, Glucose in the feed: So = 30 g/l, Plant Cell Concentration: X = 6 g/l reac. The rate constant for IA formation: k = 5 d-1 (g/l)^-1 Ks = 0.4 g/l, Column diameter: Do = 0.15 m. Growth is negligible and Monod kinetics is valid. If Yp/s is 0.02 g IA/g glu, determine IA concentration in the effluent.

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TLS Online TPP Program

#Question id: 14262

#Section 5: Bioprocess Engineering and Process Biotechnology

The BOD5 value of a waste-water feed stream to an activated-sludge unit is S0 = 300 mg/l, and the effluent is desired to be S = 30 mg/l. The feed flow rate is F = 2× 10^7 l/day. For the recycle ratio of α = 0.5 and a steady-state biomass concentration of X = 5 g/l, Use the following kinetic parameters:

 

calculate Required reactor volume (V). 

_______________10^6

TLS Online TPP Program

#Question id: 14263

#Section 5: Bioprocess Engineering and Process Biotechnology

The BOD5 value of a waste-water feed stream to an activated-sludge unit is S0 = 300 mg/l, and the effluent is desired to be S = 30 mg/l. The feed flow rate is F = 2× 10^7 l/day. For the recycle ratio of α = 0.5 and a steady-state biomass concentration of X = 5 g/l, Use the following kinetic parameters:

 

calculate Biomass concentration in recycle (Xr).

TLS Online TPP Program

#Question id: 14264

#Section 5: Bioprocess Engineering and Process Biotechnology

The BOD5 value of a waste-water feed stream to an activated-sludge unit is S0 = 300 mg/l, and the effluent is desired to be S = 30 mg/l. The feed flow rate is F = 2× 10^7 l/day. For the recycle ratio of α = 0.5 and a steady-state biomass concentration of X = 5 g/l, Use the following kinetic parameters:

 

calculate Hydraulic residence time

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TLS Online TPP Program

#Question id: 14265

#Section 5: Bioprocess Engineering and Process Biotechnology

For the activated-sludge, the specific growth rate of cells is given by 

 

The following parameter values are known: F = 500 l/h, α = 0.4, ɣ = 0.1, Xe = 0, V = 1500 l, Ks = 10 mg/l, µm = 1 h-1 , kd = 0.05 h-1 , S0 = 1000 mg/l, Y M X/S = 0.5 g dw/g substrate. Calculate the substrate concentration (S) in the reactor at steady state. -------------------------