TLS Online TPP Program

#Question id: 14237


A genetically-engineered strain of yeast is cultured in a bioreactor at 30⁰C for production of heterologous protein. The oxygen requirement is 80 mmol 1-1 h-I; the critical oxygen concentration is 0.004 mM. The solubility of oxygen in the fermentation broth is estimated to be 10% lower than in water due to solute effects. What is the minimum mass-transfer coefficient necessary to sustain this culture if the reactor is sparged with air at approximately 1 atm pressure ?         

#Section 5: Bioprocess Engineering and Process Biotechnology
  1. 0.10s-1    
  2. 0.24 s-1
  3. 0.50-1   
  4. 0.54s-1