Ethanol is produced by anaerobic fermentation of glucose by Saccharomyces cerevisiae. For the particular strain of S. cerevisiae employed, the maintenance coefficient is 0.18 kg kg^{- 1} h^{- 1}, Yxs is 0.11 kg kg^-1, Y_PX is 3.9 kg kg^-1 and µ_max is 0.4 h^-1.  It is decided to investigate the possibility of using Zymomonas mobilis bacteria instead of yeast for making ethanol. Z. mobilis is known to produce ethanol under anaerobic conditions using a different metabolic pathway to that employed by yeast. Typical values of Yxs are lower than for yeast at about 0.06 kg kg^-1; on the other hand, the maintenance coefficient is higher at 2.2 kg kg ^-1 h ^-1. Ypx for Z. mobilis is 7.7 kg kg^-l; µ_max is 0.3 h- 1.  From stoichiometry, what is the maximum theoretical yield of ethanol from glucose                 

TLS Online TPP Program

#Question id: 15477

#Section 6: Plant, Animal and Microbial Biotechnology

Why these metabolites are manufactured by microbial fermentation rather than chemical synthesis 

TLS Online TPP Program

#Question id: 15478

#Section 6: Plant, Animal and Microbial Biotechnology

Escherichia coli preferentially utilizes glucose by preventing the uptake of lactose and switching off transcription of the lac operon. This mechanism is known as 

TLS Online TPP Program

#Question id: 15479

#Section 6: Plant, Animal and Microbial Biotechnology

Out of the following what usually supports the fastest growth rate and is therefore considered the preferred nitrogen source for E. coli

TLS Online TPP Program

#Question id: 15480

#Section 6: Plant, Animal and Microbial Biotechnology

Feedback repression involves the turning “off” of enzyme synthesis when the amount of the product has been made in sufficient quantities. The end product of the pathway acts as

TLS Online TPP Program

#Question id: 15481

#Section 6: Plant, Animal and Microbial Biotechnology

One of the approaches for the bacterial strain improvement for primary metabolite include

TLS Online TPP Program

#Question id: 15482

#Section 6: Plant, Animal and Microbial Biotechnology

Over expression of primary metabolites are often achieved by

1. Frequency of RNA polymerase binding to the promoter region

2. Copy number increase of structural genes for these enzymes by genetic engineering

3. Chemically derived protein

Which combination below represent correct option?