Ethanol is produced by anaerobic fermentation of glucose by Saccharomyces cerevisiae. For the particular strain of S. cerevisiae employed, the maintenance coefficient is 0.18 kg kg^{- 1} h^{- 1}, Yxs is 0.11 kg kg^-1, Y_PX is 3.9 kg kg^-1 and µ_max is 0.4 h^-1.  It is decided to investigate the possibility of using Zymomonas mobilis bacteria instead of yeast for making ethanol. Z. mobilis is known to produce ethanol under anaerobic conditions using a different metabolic pathway to that employed by yeast. Typical values of Yxs are lower than for yeast at about 0.06 kg kg^-1; on the other hand, the maintenance coefficient is higher at 2.2 kg kg ^-1 h ^-1. Ypx for Z. mobilis is 7.7 kg kg^-l; µ_max is 0.3 h- 1. Y’_PS is maximum and equal to the theoretical yield when there is zero growth and all substrate entering the cell is used for maintenance activities. If ethanol is the sole extracellular product of energy-yielding metabolism, calculate mp for yeast?

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TLS Online TPP Program

#Question id: 15875

#Section 6: Plant, Animal and Microbial Biotechnology

Suspension cultures show both numerical and structural chromosomal changes such as;

TLS Online TPP Program

#Question id: 15876

#Section 6: Plant, Animal and Microbial Biotechnology

The following growth regulators (GRs)- auxins, cytokinins, ABA and GA3 are used in plant tissue culture, The common range of concentrations used are as follows:

GROWTH REGULATOR(GRs)                                     CONCENTERATION

A) AUXIN                                                                       i) 0.1-3 mg/L

B) GA3                                                                          ii) 0.2 mg/L

C) CYTOKININ                                                           iii) 0.1-1 mg/L

D) ABA

TLS Online TPP Program

#Question id: 15877

#Section 6: Plant, Animal and Microbial Biotechnology

Zinc as micronutrient is used in culture media for____

TLS Online TPP Program

#Question id: 15878

#Section 6: Plant, Animal and Microbial Biotechnology

A vitamin which has been found to inhibit callus formation and improve growth and quality of shoots.

TLS Online TPP Program

#Question id: 15879

#Section 6: Plant, Animal and Microbial Biotechnology

During which Phase lasts about 3-4 cell generations (the time taken for doubling of cell number), and the duration of a cell generation may vary from 22-48 hr, depending mainly on the plant species?

TLS Online TPP Program

#Question id: 15880

#Section 6: Plant, Animal and Microbial Biotechnology

 Which one types of culture is ideal for the determination of effects of individual nutrients on cell growth and metabolism.