Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the height of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

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#Question id: 27360

#Section 5: Bioprocess Engineering and Process Biotechnology

The same culture (with the m and K, values as computed above) is cultivated in a 10 L chemostat being operated with a 50 mL min-1 sterile feed containing 50 g L-1 of substrate. Assuming an overall yield coefficient of 0.3 (g- DCW) (g- substrate), the respective values of the outlet biomass and substrate concentrations are

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#Question id: 27361

#Section 5: Bioprocess Engineering and Process Biotechnology

A continuous stirred tank bioreactor produces 48 kg lysine.day-1. If the volumetric productivity is 2 g.l-1.h-1, the volume of the reactor is:

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#Question id: 27362

#Section 5: Bioprocess Engineering and Process Biotechnology

Continuous stirred tank fermenter is characterized by (biomass concentration X kg/m3, residual substrate is S kg/m3, Sf=substrate concentration in feed kg/m3, Dilution rate = D and µm>>D and Km<Sf, XD= the biomass productivity kg/m3h. If the dilution rate is doubled to 2D then the new steady state values in comparison with earlier steady state is characterized by

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#Question id: 27363

#Section 3: Genetics, Cellular and Molecular Biology

Match the types of RNA in Group I with their corresponding function in Group II

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#Question id: 27364

#Section 3: Genetics, Cellular and Molecular Biology

Which statement best describes the distinction between the purposes of the two RNAi pathways within the eukaryotic cell?

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#Question id: 27365

#Section 3: Genetics, Cellular and Molecular Biology

During siRNA-mediated gene silencing within the homo sapiens cell, the RNA-induced Silencing Complex (RISC) is composed of which of the following protein arrangements?