Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  If Yp/s is 4 mg Mab/g glu, determine the effluent Mab concentration of the system?

TLS Online TPP Program

#Question id: 13123

#Section 3: Genetics, Cellular and Molecular Biology

Match the correct one?

A- COPI                           i- vesicles transport proteins from the ER to the Golgi.

B- COPII                          ii- vesicles mainly transport proteins in the retrograde direction between Golgi cisternae and from the cis -Golgi back to the ER.

C- Clathrin                      iii- vesicles transport proteins from the plasma membrane (cell  surface) and the trans-Golgi network to late endosomes.

TLS Online TPP Program

#Question id: 13153

#General Aptitude

The in-radius of an equilateral triangle is of length 3 cm. Then the length of each of its medians is

TLS Online TPP Program

#Question id: 13156

#Section 3: Genetics, Cellular and Molecular Biology

Tay sach;s disease are caused when which enzyme is mutated or non-functional?

TLS Online TPP Program

#Question id: 13198

#Section 6: Plant, Animal and Microbial Biotechnology

The sugar is a carbohydrate that are generally translocated  in the phloem in which form?

TLS Online TPP Program

#Question id: 13199

#Section 6: Plant, Animal and Microbial Biotechnology

The non-reducing sugar sucrose is most commonly translocated in the phloem rather than reducing sugar. Why?

a.) because they are less reactive than their reducing counterparts

b.) because sucrose contains more energy than a monosaccharides

c.) because the ketone or aldehyde group is reduced to an alcohol or combined with a similar group on another sugar so as not to be oxidized 

Which one of the following statements is true about translocation of non-reducing sugar?

TLS Online TPP Program

#Question id: 13200

#Section 6: Plant, Animal and Microbial Biotechnology

In the modification of translocation pathways, Interference with a translocation pathway by wounding or pruning can alter the patterns established by proximity and vascular connections. In the absence of direct connections between source and sink, vascular interconnections, called