Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  If Yp/s is 4 mg Mab/g glu, determine the effluent Mab concentration of the system?

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#Question id: 14370

#Section 1: Engineering Mathematics

The value of x, if

 is

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#Question id: 10515

#Section 6: Plant, Animal and Microbial Biotechnology

Insect-derived elicitors is,

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#Question id: 3881

#Section 3: Genetics, Cellular and Molecular Biology

Which one of the following statements about mRNA stability is true?

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#Question id: 3638

#Section 3: Genetics, Cellular and Molecular Biology

Four different mutant lines showing similar phenotype were identified from a genetic screen. When genetic crosses among these mutants were carried out, the first mutant was found to complement the second, third and fourth mutant lines. However, no other complementation groups do the four mutant lines belongs to?

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#Question id: 12948

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Proteolytic cleavage of DNA polymerase by subtilisin caused loss of its nick translation activity. Which of the following properties is lost?