Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  If Yp/s is 4 mg Mab/g glu, determine the effluent Mab concentration of the system?

TLS Online TPP Program

#Question id: 15897

#Section 6: Plant, Animal and Microbial Biotechnology

Primary somatic embryo regenerating from the

TLS Online TPP Program

#Question id: 15898

#Section 6: Plant, Animal and Microbial Biotechnology

Which of the following is not related to embryo culture?

TLS Online TPP Program

#Question id: 15899

#Section 6: Plant, Animal and Microbial Biotechnology

Somatic embryos (SEs) regenerate from the tissues of other SEs or the parts of germinating SEs; such SEs are called

TLS Online TPP Program

#Question id: 15900

#Section 6: Plant, Animal and Microbial Biotechnology

During development of a somatic embryo from a single superficial cell of the explant, the cells of this meristematic mass continue to divide to give rise to the various stages of SEs. Which of the following is considered correct for the development of SEs?

TLS Online TPP Program

#Question id: 15901

#Section 6: Plant, Animal and Microbial Biotechnology

The following features are included between the shoot buds and somatic embryo;

                                                                             FEATURES

A) SHOOT BUD                                 i) Originated from the many cells, usually superficial

B) SOMATIC EMBRYO                        ii) Polarity is defined as bipolar; both shoot and root poles present

                                                        iii) Vascular strands connected with those present in callus/explant

                                                       iv) Easily separated from the callus/explant

                                                        v) There is no vascular connection with callus/explant

Which of the following correctly represents the features of  shoot buds and somatic embryo?

TLS Online TPP Program

#Question id: 15902

#Section 6: Plant, Animal and Microbial Biotechnology

The pair of hormones required for a callus to differentiate are____