C. roseus cells immobilized in Ca-alginate beads of diameter 0.5 mm are used for production of indole alkaloids (IA) in a fluidized-bed bioreactor. The rate limiting nutrient is glucose and no intraparticle diffusion limitations exist. Use the following data: Flow rate of the feed: Q = 1 l/h, Glucose in the feed: So = 30 g/l, Plant Cell Concentration: X = 6 g/l reac. The rate constant for IA formation: k = 5 d-1 (g/l)^-1 Ks = 0.4 g/l, Column diameter: Do = 0.15 m. Growth is negligible and Monod kinetics is valid. For 95% glucose conversion determine volume. 

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#Question id: 12912

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the enzyme which separates the double-helical DNA into single strands.

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#Question id: 14172

#Section 4: Fundamentals of Biological Engineering

The growth of baker’s yeast (S. cerevisiae) on glucose may be simply described by the following equation:

In a batch reactor of volume 105 l, the final desired yeast concentration is 50 gdw/l. Using the above reaction stoichiometry. Determine the yield coefficients YX/S (biomass/glucose)?

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#Question id: 4156

#Section 3: Genetics, Cellular and Molecular Biology

The reading frame of DNA is fixed by

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#Question id: 569

#Section 2: General Biology

A small molecule that decreases the activity of an enzyme by binding to a site other than the catalytic site is termed a(n):

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#Question id: 3250

#Section 3: Genetics, Cellular and Molecular Biology

Consider a diploid population at Hardy-Weinberg equilibrium. For a locus with two alleles, the frequency of the A1A1, A1A2 and A2A2 genotype is 0.01, 0.18 and 0.81) Which of the following statement is correct?