C. roseus cells immobilized in Ca-alginate beads of diameter 0.5 mm are used for production of indole alkaloids (IA) in a fluidized-bed bioreactor. The rate limiting nutrient is glucose and no intraparticle diffusion limitations exist. Use the following data: Flow rate of the feed: Q = 1 l/h, Glucose in the feed: So = 30 g/l, Plant Cell Concentration: X = 6 g/l reac. The rate constant for IA formation: k = 5 d-1 (g/l)^-1 Ks = 0.4 g/l, Column diameter: Do = 0.15 m. Growth is negligible and Monod kinetics is valid. For 95% glucose conversion determine height of the column 

#Question id: 14233

#Section 5: Bioprocess Engineering and Process Biotechnology

Nutrient medium is to be heated from 10⁰C to 28⁰C is a single-pass countercurrent shell-and-tube heat exchanger before being pumped into a fed-batch fermenter. Medium passes through the tubes of the exchanger; the shell-side fluid is water which enters with flow rate 3 x 10^4 kg h^- 1 and temperature 60⁰C pre-heated medium is required at a rate of 50 m^3 h^-1. The density, viscosity and heat capacity of the medium are the same as water; the thermal conductivity of the medium is 0.54 Wm^- 1 ⁰C ^- 1. It is proposed to use 30 steel tubes with inner diameter 5 cm; the tubes will be arranged in line. The pipe wall is 5-mm thick; the thermal conductivity of the metal is 50 Wm^- 1 ⁰C ^- 1. The maximum linear shell-side fluid velocity is estimated as 0.15 m s^-1. Calculate the overall heat-transfer coefficient._________

#Question id: 14234

#Section 5: Bioprocess Engineering and Process Biotechnology

Nutrient medium is to be heated from 10⁰C to 28⁰C is a single-pass countercurrent shell-and-tube heat exchanger before being pumped into a fed-batch fermenter. Medium passes through the tubes of the exchanger; the shell-side fluid is water which enters with flow rate 3 x 10^4 kg h^- 1 and temperature 60⁰C pre-heated medium is required at a rate of 50 m^3 h^-1. The density, viscosity and heat capacity of the medium are the same as water; the thermal conductivity of the medium is 0.54 Wm^- 1 ⁰C ^- 1. It is proposed to use 30 steel tubes with inner diameter 5 cm; the tubes will be arranged in line. The pipe wall is 5-mm thick; the thermal conductivity of the metal is 50 Wm^- 1 ⁰C ^- 1. The maximum linear shell-side fluid velocity is estimated as 0.15 m s^-1. Calculate the log-mean temperature difference _________

#Question id: 14235

#Section 5: Bioprocess Engineering and Process Biotechnology

Nutrient medium is to be heated from 10⁰C to 28⁰C is a single-pass countercurrent shell-and-tube heat exchanger before being pumped into a fed-batch fermenter. Medium passes through the tubes of the exchanger; the shell-side fluid is water which enters with flow rate 3 x 10^4 kg h^- 1 and temperature 60⁰C pre-heated medium is required at a rate of 50 m^3 h^-1. The density, viscosity and heat capacity of the medium are the same as water; the thermal conductivity of the medium is 0.54 Wm^- 1 ⁰C^ - 1. It is proposed to use 30 steel tubes with inner diameter 5 cm; the tubes will be arranged in line. The pipe wall is 5-mm thick; the thermal conductivity of the metal is 50 Wm^- 1 ⁰C^ - 1. The maximum linear shell-side fluid velocity is estimated as 0.15 m s^-1.  Determine the heat-transfer area. ____________

#Question id: 14236

#Section 5: Bioprocess Engineering and Process Biotechnology

#Question id: 14237

#Section 5: Bioprocess Engineering and Process Biotechnology

A genetically-engineered strain of yeast is cultured in a bioreactor at 30⁰C for production of heterologous protein. The oxygen requirement is 80 mmol 1-1 h-I; the critical oxygen concentration is 0.004 mM. The solubility of oxygen in the fermentation broth is estimated to be 10% lower than in water due to solute effects. What is the minimum mass-transfer coefficient necessary to sustain this culture if the reactor is sparged with air at approximately 1 atm pressure ?         

#Question id: 14238

#Section 5: Bioprocess Engineering and Process Biotechnology

A genetically-engineered strain of yeast is cultured in a bioreactor at 30⁰C for production of heterologous protein. The oxygen requirement is 80 mmol 1-1 h-I; the critical oxygen concentration is 0.004 mM. The solubility of oxygen in the fermentation broth is estimated to be 10% lower than in water due to solute effects. What mass-transfer coefficient is required if pure oxygen is used instead of air?