In a trickling biological filter, the BOD value of the feed stream is S_0i = 500 mg/l with a feed flow of F = 10³ l/h. The effluent BOD value is desired to be S₀ = 10 mg/l. The following kinetic parameters for the biocatalysts are known: r_m = 20 mg/S/l .h and K_s = 200 mg S/l. The biofilm thickness is L = 0.1 mm. The cross-sectional area of the filter is A = 2 m² , and the biofilm surface area per unit volume of the bed is a = 500 cm² /cm³ . Assume that dissolved oxygen is the rate-limiting substrate and the diffusion coefficient of oxygen is D_O2 = 2 × 10^-5 cm² /s. Determine the required height of the bed. You can assume first-order bioreaction kinetics?

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TLS Online TPP Program

#Question id: 14394

#Section 5: Bioprocess Engineering and Process Biotechnology

1.0 timol of an enzyme that contains five active sites per molecule converts 1.0 pmol substrate every 2 microseconds. Calculate catalytic centre activity (sec^-1). ......10^5

TLS Online TPP Program

#Question id: 14395

#Section 5: Bioprocess Engineering and Process Biotechnology

An enzyme is available at a specific activity of 500 units/mg protein. For an assay, 10miro mole substrate need to be converted per minute. What mass (in mg) of protein would you take for this to be achieved? 

TLS Online TPP Program

#Question id: 14396

#Section 5: Bioprocess Engineering and Process Biotechnology

A purified hydrolytic enzyme (Mr 150 000) is available with the description: Gives a Vmax of 3 200 units/mg protein under optimal conditions. How many micromoles of substrate can be hydrolysed by 1 mg of protein in 1 minute  

TLS Online TPP Program

#Question id: 14397

#Section 5: Bioprocess Engineering and Process Biotechnology

A purified hydrolytic enzyme (Mr 150 000) is available with the description: Gives a Vmax of 3 200 units/mg protein under optimal conditions. How many nmol of enzyme are there in 1 mg  

TLS Online TPP Program

#Question id: 14398

#Section 5: Bioprocess Engineering and Process Biotechnology

The internal effectiveness factor is useful because , which statement is not correct 

TLS Online TPP Program

#Question id: 14399

#Section 5: Bioprocess Engineering and Process Biotechnology

 observable Thiele modulus CD decreases when