Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the concentration of cells at quasi-steady state when t = 10 h.

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#Question id: 20416

#Section 5: Bioprocess Engineering and Process Biotechnology

Which of the following would be correct concerning the concentration of biomass (X). growth limiting substrate (S) and product (P) in a chemostat when the cells have been washed out?

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#Question id: 555

#Section 2: General Biology

The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by To determine Km from a double-reciprocal plot, you would:

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#Question id: 27910

#Section 5: Bioprocess Engineering and Process Biotechnology

The rate of oxygen tranfer is given by equation 

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#Question id: 16026

#Section 6: Plant, Animal and Microbial Biotechnology

At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

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#Question id: 3610

#Section 3: Genetics, Cellular and Molecular Biology

If the genotype consists of only one type of allele. It is called