TLS Online TPP Program

#Question id: 14272


Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. If qP = 0.05 g product/g cells h and P0 = 0.1 g/l, determine the product concentration in the vessel at t = 10 h

#Section 5: Bioprocess Engineering and Process Biotechnology
  1. 20.7g/l 
  2. 16.875g/l      
  3. 22.5g/l      
  4. 15.6g/l
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TLS Online TPP Program

#Question id: 14279

#Section 5: Bioprocess Engineering and Process Biotechnology

Growth yield of mDicrobes is defined as

TLS Online TPP Program

#Question id: 3579

#Section 3: Genetics, Cellular and Molecular Biology

Following some statement is correct with monohybrid and Dihybrid cross?

A) Two alleles for gene segregate into gametes in equal proportions.

B) Monohybrid F2 phenotype 1:2:1 ratio conclude, there is no segregation in character

C)  Monohybrid F2 phenotype 3:1 ratio conclude, there is a no blending inheritance

D) Dihybrid F 2 phenotype 3:1 ratio conclude complete linkage

TLS Online TPP Program

#Question id: 15890

#Section 6: Plant, Animal and Microbial Biotechnology

 Tissue culture was developed by _______ but the concept of tissue culture was conceived by ______

TLS Online TPP Program

#Question id: 12909

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

According to the diagram, arrange the steps in correct order: 


1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 
2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 
3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).
 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

TLS Online TPP Program

#Question id: 14333

#Section 5: Bioprocess Engineering and Process Biotechnology

A cylindrical bioreactor of diameter 3 m has four baffles. A Rushton turbine mounted in the reactor has a diameter one third the tank diameter and is operated at a speed of 90 rpm. The density of the fluid is approximately I g cm-3. The reactor is used to culture an anaerobic organism that does not require gas sparging. The broth can be assumed Newtonian. As the cells grow, the viscosity of the broth increases. when the viscosity is 1000 times greater than water, estimate the power required to achieve turbulence.